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Updated: Jul 20, 2026

In Vitro Assay to Measure Phosphatidylethanolamine Methyltransferase Activity
Published on: January 5, 2016
Posttranscriptional regulation by the upstream open reading frame of the phosphoethanolamine N-methyltransferase gene
Tomoki Tabuchi1, Tomoyuki Okada, Tetsushi Azuma
1Laboratory of Tropical Plant Science, Faculty of Agriculture, Kobe University, Kobe, Japan. nb_totabuchi@nara.kindai.ac.jp
Abstract:
Phosphoethanolamine N-methyltransferase (PEAMT) is involved in choline biosynthesis in plants. The 5' untranslated region (UTR) of several PEAMT genes was found to contain an upstream open reading frame (uORF). We generated transgenic Arabidopsis calli that expressed a chimeric gene constructed by fusing the 5' UTR of the Arabidopsis PEAMT gene (AtNMT1) upstream of the beta-glucuronidase gene. The AtNMT1 uORF was found to be involved in declining levels of the chimeric gene mRNA and repression of downstream beta-glucuronidase gene translation in the calli when the cells were treated with choline. Further, we discuss the role of the uORF.
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