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Updated: Jul 20, 2026

Spectrophotometric Determination of Phycobiliprotein Content in Cyanobacterium Synechocystis
Published on: September 11, 2018
[Spectrophotometric determination of chondroitin sulfate with victory blue B]
Yuan-yuan Chen1, Zhi-liang Jiang, Zhen-zhong Li
1Department of Resource and Environmental Science, Guangxi Normal University, Guilin, China.
Abstract:
In pH 4.0 acetic acid-sodium acetate buffer solution, cationic dye victory blue B shows an absorption peak at 614 nm, and the absorption peak decreases after it reacts with chondroitin sulfate to form association particles. The decrease in absorption value is linear with chondroitin sulfate concentration in the range of 0. 1-5 microg x mL(-1), and the correlation efficient is 0.9986. The method was applied to the determination of chondroitin sulfate in synthesis samples and real samples with rapidity, simplicity and good accuracy.
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