Related Experiment Video
Updated: Oct 12, 2026

Identification and Characterization of Protein Glycosylation using Specific Endo- and Exoglycosidases
Published on: December 26, 2011
[Labeled histochemical localization of carbohydrate components of glycoproteins: glucose-6-phosphate and
Abstract:
In the present work methods for the localization of glucose-6-phosphate and phosphoenolpyruvate residues on tissue sections by means of labeled with colloidal gold specific enzymes (glucose 6-phosphate dehydrogenase and pyruvate kinase) are described. In order to get sufficient amount of labeled enzyme to the protein salts, used to stabilize colloidal gold salts, albumin was added. Residues of glucose-6-phosphoenolpyruvate were scattered equally through the villi of human placenta. In rat liver centrolobular localized hepatocytes had high content of specific staining. There were a lot of glucose-6-phosphate residues in hepatocytes nuclei.
Related Concept Videos
What is Glycolysis?
Cells make energy by breaking down macromolecules. Cellular respiration is the biochemical process that converts "food energy" (from the chemical bonds of macromolecules) into chemical energy in the form of adenosine triphosphate (ATP). The first step of this tightly regulated and intricate process is glycolysis. The word glycolysis originates from the Latin glyco (sugar) and lysis (breakdown). Glycolysis serves two main intracellular functions: generating ATP and generating...
Membrane Carbohydrates
Membrane carbohydrates do not have any hydrophobic region and are exclusively located on the cell's outer surface. The addition of sugar molecules or glycosylation of proteins happens in...
Protein Glycosylation
Glycosylation occurs in...
Labeling DNA Probes
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...
Glycolysis: Preparatory Phase
Biosynthesis of Polysaccharides

