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Cleavage of tensin during cytoskeleton disruption in YTX-induced apoptosis

Mónica Suárez Korsnes1, Dyveke Lem Hetland, Arild Espenes

  • 1The Norwegian School of Veterinary Science, P.O. Box 8146, Dep. No. 0033, Food Safety and Infection Biology Department, Ullevaalsveien 72, Oslo, Norway. Monica.Suarez@veths.no

Toxicology in Vitro : an International Journal Published in Association with BIBRA
|September 12, 2006
PubMed

Insights

Yessotoxin (YTX) disrupts the F-actin cytoskeleton and cleaves tensin, a key protein in cell adhesion and shape maintenance. This marine toxin alters myoblast cell structure, leading to potential cell shape changes and lamella formation.

Area of Science:

  • Cell Biology
  • Toxicology
  • Biochemistry

Background:

  • Yessotoxin (YTX) is a marine algal toxin.
  • YTX induces apoptosis in myoblast cell lines.
  • YTX causes F-actin cytoskeleton disassembly and tensin cleavage.

Purpose of the Study:

  • To evaluate F-actin cytoskeleton disruption by YTX.
  • To assess changes in tensin protein in myoblast cell lines.
  • To understand YTX's mechanism of action on cell structure.

Main Methods:

  • Exposure of L6 and BC3H1 myoblast cell lines to 100 nM YTX.
  • Incubation periods up to 72 hours.
  • Analysis of F-actin cytoskeleton and tensin protein localization and cleavage.

Main Results:

  • YTX treatment cleaves tensin, causing its translocation to the cell center.
  • YTX alters cell shape and induces lamella formation associated with pseudopodia.
  • Disruption of the F-actin cytoskeleton and tensin is confirmed.

Conclusions:

  • YTX significantly impacts myoblast cell structure by disrupting the F-actin cytoskeleton and altering tensin.
  • Tensin cleavage and translocation by YTX suggest a role in YTX-induced apoptosis and cell shape changes.
  • Further research is needed to identify alternative proteins involved in focal contact anchoring after YTX exposure.

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