Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

A novel fluorescence-based cellular permeability assay.

Ankur Chandra1, Samuel Barillas, Ahmed Suliman

  • 1Section of Vascular and Endovascular Surgery, Department of Surgery, Univeristy of California, San Diego Medical Center, 200 West Arbor Drive, San Diego, CA 92103-8402, USA.

Journal of Biochemical and Biophysical Methods
|September 12, 2006
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Drug-Induced Cholestasis Secondary to Meropenem in an Elderly Patient: Report of A Rare Case.

Cureus·2026
Same author

Laparoscopic Repair of Diaphragmatic Pericardial Hernia Following Blunt Trauma: A Case Report.

The American journal of case reports·2025
Same author

A Case Report on Schistosoma and Gastric Cancer: Association or Causation?

Cureus·2025
Same author

Management of Plastic Surgery Complications at a Tertiary Medical Center after Aesthetic Procedures.

Plastic and reconstructive surgery. Global open·2024
Same author

Bilateral internal carotid artery hypoplasia presenting with watershed territory infarcts.

Radiology case reports·2024
Same author

Greater Disease Severity and Worse Clinical Outcomes in Patients Hospitalised with COVID-19 in Africa.

Global heart·2024
Same journal

Proceedings of the 12th International Symposium Of Chemistry, Miercurea Ciuc (Csíkszereda), Romania, October 5-8, 2006; and proceedings of the 7th International Symposium and Summer School on Bioanalysis, Pécs, Hungary, June 10-17, 2007.

Journal of biochemical and biophysical methods·2008
Same journal

12th International Symposium Of Chemistry, Miercurea Ciuc (Csíkszereda), Romania, 5th to 8th of October, 2006. 7th International Symposium and Summer School on Bioanalysis, Pécs, Hungary, 10th to 17th of June, 2007.

Journal of biochemical and biophysical methods·2008
Same journal

Optimized HPLC method for tramadol and O-desmethyl tramadol determination in human plasma.

Journal of biochemical and biophysical methods·2008
Same journal

The alternating current polarographic behavior and determination of lansoprazole and omeprazole in dosage forms and biological fluids.

Journal of biochemical and biophysical methods·2008
Same journal

Continuous-flow complete-mixing system for assessing the effects of environmental factors on colony-level coral metabolism.

Journal of biochemical and biophysical methods·2008
Same journal

Utilization of fluorescence tracer in hyperinsulinemic-euglycemic clamp test in mice.

Journal of biochemical and biophysical methods·2008
See all related articles

This study introduces a novel fluorescence-based assay to measure vascular permeability. The assay quantifies changes in cell layer permeability without radioactivity, offering a simpler and safer method for disease research.

Area of Science:

  • Biomedical Engineering
  • Cell Biology
  • Vascular Biology

Background:

  • Vascular permeability is a critical factor in diseases like cancer metastasis and ischemia-reperfusion injury.
  • Existing methods for measuring permeability often involve radioactivity or electrical impedance, posing safety and complexity challenges.
  • Precise quantification of cell layer permeability is essential for understanding various pathological processes.

Purpose of the Study:

  • To develop a novel, sensitive, and safe fluorescence-based assay for quantifying vascular permeability.
  • To measure the trans-layer flux across human aortic endothelial cell monolayers.
  • To establish an in vitro method for studying cellular permeability dynamics in pathological conditions.

Main Methods:

  • Human aortic endothelial cells were cultured as monolayers on Transwell inserts.

Related Experiment Videos

  • Vascular Endothelial Growth Factor (VEGF(165)) was used to stimulate permeability.
  • Fluorescent microspheres were employed to quantify trans-layer flux, replacing radioactive tracers or impedance measurements.

    • Main Results:

    • The fluorescence-based assay detected dose-dependent increases in trans-layer flux upon VEGF(165) stimulation.
    • Significant increases in permeability were observed at VEGF(165) concentrations of 20, 40, 80, and 100 ng/ml.
    • The assay demonstrated sensitivity to fluid flux changes independent of protein flux.

    Conclusions:

    • A novel, sensitive, and safer fluorescence-based assay for measuring cell layer permeability has been established.
    • This assay provides a valuable tool for investigating the physiology and pathology of vascular permeability.
    • The method's simplicity and safety facilitate broader application in disease research.