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Identification of cell lines permissive for human coronavirus NL63

Oliver Schildgen1, Maarten F Jebbink, Michel de Vries

  • 1University of Bonn, Institute for Medical Microbiology, Immunology, and Parasitology, Department of Virology, and Children's Hospital, Sigmund-Freud-Strasse 25, D-53105 Bonn, Germany. schildgen@mibi03.meb.uni-bonn.de

Journal of Virological Methods
|September 12, 2006
PubMed

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Cell Lines

A cell line is a population of cells grown in vitro that can be subcultured over several generations. Normal cells cease to divide after a certain number of cell divisions, a process known as replicative senescence. This number, called the Hayflick limit, was conceptualized by Leonard Hayflick in 1961 when he observed that fetal cells grown in culture could only divide 40-60 times. This limit is due to the shortening of the telomeres during each round of cell division, preventing cell division...