Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Spermatogenesis01:41

Spermatogenesis

Spermatogenesis is the process by which haploid sperm cells are produced in the male testes. It starts with stem cells located close to the outer rim of seminiferous tubules. These spermatogonial stem cells divide asymmetrically to give rise to additional stem cells (meaning that these structures “self-renew”), as well as sperm progenitors, called spermatocytes. Importantly, this method of asymmetric mitotic division maintains a population of spermatogonial stem cells in the male reproductive...
Spermatogenesis01:22

Spermatogenesis

Spermatogenesis is a complex process that involves the development of sperm cells from undifferentiated stem cells in the seminiferous tubules of the testes. The process is essential for the production of mature and functional sperm cells that are capable of fertilizing an egg.
The process of spermatogenesis can be divided into mitosis, meiosis, and spermiogenesis. During mitosis, the spermatogonia or stem cells divide to produce two identical daughter cells, type A and B spermatogonia. Type-A...
Meiosis I03:09

Meiosis I

Meiosis is the division of a diploid cell into haploid cells forming sperm and eggs in animals through differentiation. Meiosis I is the first stage of meiosis, where the genetic recombination of homologous chromosomes and the reduction of the ploidy level by half occurs.
Prophase I is the most extended and complex step of meiosis I characterized by synapsis, chromosome pairing, and recombination of the homologous chromosomes. This process is facilitated by a proteinaceous structure called the...
Meiosis I01:49

Meiosis I

Meiosis is a carefully orchestrated set of cell divisions, the goal of which—in humans—is to produce haploid sperm or eggs, each containing half the number of chromosomes present in somatic cells elsewhere in the body. Meiosis I is the first such division, and involves several key steps, among them: condensation of replicated chromosomes in diploid cells; the pairing of homologous chromosomes and their exchange of information; and finally, the separation of homologous chromosomes by a...
Meiosis II01:57

Meiosis II

Meiosis II is the second and final stage of meiosis. It relies on the haploid cells produced during meiosis I, each of which contain only 23 chromosomes—one from each homologous initial pair. Importantly, each chromosome in these cells is composed of two joined copies, and when these cells enter meiosis II, the goal is to separate such sister chromatids using the same microtubule-based network employed in other division processes. The result of meiosis II is two haploid cells, each containing...
Nondisjunction01:21

Nondisjunction

Nondisjunction is the failure of homologous chromosomes or sister chromatids to separate correctly and move to the opposite poles of the cells. This produces daughter cells with abnormal chromosome numbers.  Nondisjunction is common during anaphase I or anaphase II of meiosis.  Mutations in synaptonemal complex proteins that attach homologous chromosomes increase the chances of nondisjunction in anaphase I of meiosis I. In contrast, mutations in topoisomerases and condensins that hold sister...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

<i>GripAgain</i> - Development of a Mobile Application (App) for hand function training after nerve surgery.

Brain & spine·2026
Same author

Effectiveness of a home-based training program on hand function after carpal tunnel release: A prospective multicenter trial.

Brain & spine·2026
Same author

Abstracts International Scientific Day on Oxidative Stress, Aging, and Age-Related Diseases.

La Tunisie medicale·2026
Same author

[Predictive factors of spontaneous pregnancies among women with diminished ovarian reserve patients treated with DHEA].

Gynecologie, obstetrique, fertilite & senologie·2023
Same author

Comparative effectiveness of gonadotropins used for ovarian stimulation during assisted reproductive technologies (ART) in France: A real-world observational study from the French nationwide claims database (SNDS).

Best practice & research. Clinical obstetrics & gynaecology·2023
Same author

Motivations and personality characteristics of candidate sperm and oocyte donors according to parenthood status: a national study from the French CECOS network.

Human reproduction open·2022

Related Experiment Video

Updated: Jul 20, 2026

Single Oocyte Bisulfite Mutagenesis
13:18

Single Oocyte Bisulfite Mutagenesis

Published on: June 27, 2012

[DNA sperm methylation in assisted reproductive techniques].

M Benchaïb1, M Ajina, V Braun

  • 1Département de médecine et biologie de la reproduction, hôpital Edouard-Herriot, place d'Arsonval, 69373 Lyon cedex 03, France. mehdi.benchaib@sante.univ-lyon1.fr

Gynecologie, Obstetrique & Fertilite
|September 12, 2006
PubMed
Summary

Spermatic DNA methylation levels are crucial for pregnancy success in in vitro fertilization (IVF). Low DNA methylation in sperm is linked to decreased pregnancy rates, indicating its role in male fertility and embryonic development.

More Related Videos

Rapid and Efficient Spatiotemporal Monitoring of Normal and Aberrant Cytosine Methylation within Intact Zebrafish Embryos
07:16

Rapid and Efficient Spatiotemporal Monitoring of Normal and Aberrant Cytosine Methylation within Intact Zebrafish Embryos

Published on: August 18, 2022

Immunostaining for DNA Modifications: Computational Analysis of Confocal Images
09:42

Immunostaining for DNA Modifications: Computational Analysis of Confocal Images

Published on: September 7, 2017

Related Experiment Videos

Last Updated: Jul 20, 2026

Single Oocyte Bisulfite Mutagenesis
13:18

Single Oocyte Bisulfite Mutagenesis

Published on: June 27, 2012

Rapid and Efficient Spatiotemporal Monitoring of Normal and Aberrant Cytosine Methylation within Intact Zebrafish Embryos
07:16

Rapid and Efficient Spatiotemporal Monitoring of Normal and Aberrant Cytosine Methylation within Intact Zebrafish Embryos

Published on: August 18, 2022

Immunostaining for DNA Modifications: Computational Analysis of Confocal Images
09:42

Immunostaining for DNA Modifications: Computational Analysis of Confocal Images

Published on: September 7, 2017

Area of Science:

  • Reproductive Biology
  • Epigenetics
  • Spermatozoa Function

Context:

  • Current methods for assessing sperm fertilizing properties lack prognostic value.
  • Spermatic DNA integrity is essential for successful fertilization and pregnancy.
  • DNA methylation plays a key role in regulating gene expression during embryonic development in mammals.

Purpose:

  • To evaluate the impact of spermatic DNA methylation levels on in vitro fertilization (IVF) outcomes.
  • To assess the relationship between DNA methylation and fertilization rate, embryo quality, and pregnancy rate.

Summary:

  • Spermatic DNA methylation was objectively evaluated using immunostaining of 5-methylcytosine and quantified via image analysis or flow cytometry.
  • Results indicate that DNA methylation levels do not affect fertilization rates or embryo quality.
  • A decreased pregnancy rate was observed when spermatic DNA methylation levels fell below a specific threshold.

Impact:

  • Spermatic DNA methylation emerges as a novel parameter for assessing sperm maturation.
  • This finding has implications for improving prognostic factors in assisted reproductive technologies.
  • Understanding DNA methylation's role can enhance strategies for male infertility treatment.