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Published on: August 28, 2014
Surface modification of poly(hydroxybutyrate) films to control cell-matrix adhesion
Tilo Pompe1, Kristin Keller, Gisela Mothes
1Leibniz Institute of Polymer Research Dresden, Max Bergmann Center of Biomaterials Dresden, Hohe Strasse 6, 01069 Dresden, Germany. pompe-tilo@ipfdd.de
Biomaterials
|September 12, 2006
Summary
Modifying poly(hydroxybutyrate) surfaces with plasma or alkali treatments enhances hydrophilicity and controls cell adhesion. Surface chemistry changes dictate fibronectin anchorage and endothelial cell behavior in tissue engineering scaffolds.
Area of Science:
- Biomaterials Science
- Tissue Engineering
- Surface Chemistry
Background:
- Degradable polymer scaffolds are crucial for tissue engineering.
- Controlling surface properties is essential for cell-matrix interactions.
- Poly(3-hydroxybutyrate) and its copolymers are promising biomaterials.
Purpose of the Study:
- To modify poly(hydroxybutyrate) surfaces to control endothelial cell adhesion.
- To investigate the relationship between surface physicochemical properties and cell-matrix interactions.
- To understand fibronectin heteroexchange and reorganization on modified surfaces.
Main Methods:
- Surface modification using ammonia plasma, water vapor plasma, and sodium hydroxide treatment.
- Surface characterization via X-ray photoelectron spectroscopy (XPS), scanning force microscopy (SFM), electrokinetic, and contact angle measurements.
- Evaluation of fibronectin (FN) heteroexchange and human umbilical cord vein endothelial cell adhesion over time.
Main Results:
- All treatments increased surface hydrophilicity.
- Ammonia plasma introduced amine groups, water vapor plasma introduced carboxyl groups, and NaOH caused ester hydrolysis.
- Surface modification type influenced fibronectin anchorage strength, which in turn affected cell adhesion and reorganization.
- Cell adhesion and fibronectin reorganization were sensitive to the physicochemical profile of the modified surfaces.
Conclusions:
- Surface modification of poly(hydroxybutyrate) scaffolds can effectively control endothelial cell adhesion.
- The observed effects are linked to changes in surface chemistry and hydrophilicity, influencing fibronectin interactions.
- This study provides insights into tailoring biomaterial surfaces for improved tissue engineering applications.
