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Light emitting diodes for auramine O fluorescence microscopic screening of Mycobacterium tuberculosis
R M Anthony1, A H J Kolk, S Kuijper
1Department of Biomedical Research, Royal Tropical Institute (KIT), Amsterdam, The Netherlands. r.anthony@kit.nl
Abstract:
We describe the simple adaptation of a standard fluorescent microscope for illumination using a 'Royal Blue' Luxeon light emitting diode (LED) and demonstrate that this form of illumination is suitable for the detection of auramine O stained Mycobacterium spp. The low cost, low power consumption, safety and reliability of LEDs makes them attractive alternatives to mercury vapour lamps.
