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Phosphotyrosine-containing proteins in Dictyostelium discoideum
A Schweiger1, O Mihalache, A Muhr
1Max-Planck-Institut für Biochemie, Martinsried bei München, FRG.
FEBS Letters
|July 30, 1990
Abstract:
Phosphotyrosine-containing proteins in Dictyostelium discoideum were detected by immunoblot analysis and immunoprecipitation using a monoclonal anti-phosphotyrosine antibody. The iodinated antibody recognized on bots a cluster of 205-220 kDa polypeptides and bands of 107 and 60 kDa. The 107 and 60 kDa polypeptides and, in addition, a 82 kDa one became phosphorylated on tyrosine when the immunoprecipitate was incubated with [gamma-32P]ATP. In preparations from differentiating cells the intensity of the label was increased in the 60 kDa band and decreased in the 107 and 205-220 kDa bands.