Selective and sensitive trace analysis of sulfur mustard with thermal desorption and two-dimensional gas
J P Oostdijk1, C E A M Degenhardt, H C Trap
1Varian B.V., Department R&D Consumable Products, Herculesweg 8, 4338 PL Middelburg, The Netherlands. john.oostdijk@varianinc.com
Abstract:
An improved method is presented for the trace analysis of sulfur mustard (HD) in biological samples, such as blood and tissue from laboratory animals. Using the internal standard method and liquid-liquid extraction with ethyl acetate, up to 400 microL of the extract was injected by thermal desorption from Tenax and analyzed by two-dimensional GC-MS/EI in SIM mode. The analysis was compared with a direct GC injection. Reversed thermal desorption was used as a tool for handling heavily contaminated (fat) samples, thus preventing contamination of the injection system and pre-column. A successful analytical configuration has been set up for the bioanalysis of HD at the low, toxicologically relevant pM level. A detection limit of 10 pg mL(-1) blood or pg g(-1) tissue of sulfur mustard (S/N=3) was established by using this configuration.
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