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Related Experiment Videos

A human cellular sequence implicated in trk oncogene activation is DNA damage inducible.

R Ben-Ishai1, R Scharf, R Sharon

  • 1Department of Biology, Technion-Israel Institute of Technology, Haifa, Israel.

Proceedings of the National Academy of Sciences of the United States of America
|August 1, 1990
PubMed
Summary

Researchers identified a DNA-damage-inducible gene (C-5) in human cells. Its expression increases after UV exposure, especially in cells with impaired DNA repair, suggesting a role in carcinogen response.

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Area of Science:

  • Molecular Biology
  • Genetics
  • Cell Biology

Background:

  • Xeroderma pigmentosum cells lack DNA repair mechanisms for UV damage.
  • Identifying DNA-damage-inducible transcripts is crucial for understanding cellular responses to genotoxic agents.

Purpose of the Study:

  • To clone and characterize DNA-damage-inducible transcripts in human cells.
  • To investigate the regulation and function of the identified C-5 transcript.

Main Methods:

  • Differential screening of cDNA libraries from UV-treated and untreated human cells.
  • RNA gel blot analysis to assess transcript abundance and regulation.
  • Southern blot analysis to determine gene family size.
  • Sequence analysis of the C-5 cDNA clone.

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Main Results:

  • A 1-kilobase transcript (C-5 RNA) was identified, moderately abundant in normal cells and enhanced by UV irradiation and other DNA-damaging agents.
  • UV-enhanced transcription of C-5 RNA was transient and more pronounced in DNA-repair-deficient cells.
  • The C-5 gene is part of a multigene family.
  • Sequence analysis revealed homology to the amino-terminal activating sequence of the trk-2h chimeric oncogene.

Conclusions:

  • The C-5 gene product is a DNA-damage-inducible transcript.
  • Its enhanced expression in repair-deficient cells suggests a role in cellular response to DNA damage.
  • The homology to an oncogene's activating sequence indicates potential implications in carcinogen-induced oncogenesis.