Related Experiment Video
Updated: Jul 20, 2026

Electrochemically and Bioelectrochemically Induced Ammonium Recovery
Published on: January 22, 2015
Ammonia removal from livestock wastewater by ammonia-assimilating microorganisms immobilized in polyvinyl alcohol
Hiraku Sasaki1, Jun Nonaka, Takako Sasaki
1Laboratory of Animal Health and Management, Graduate School of Agricultural Science, Tohoku University, Naruko, Miyagi, 989-6711, Japan.
Abstract:
We isolated ammonia-assimilating microorganisms from the livestock manure treatment systems and evaluated their ammonia-assimilating ability. Many isolates utilized ammonia at high rates when they were purely cultivated in a nitrogen-limited medium to which sterilized lagoon extract had been added. Some isolates that were immobilized in polyvinyl alcohol (PVA) utilized ammonia present in the media containing viable lagoon microorganisms. Staining with 4',6'-diamidino-2-phenylindole (DAPI) indicated that the immobilized high ammonia-assimilating isolates grew dominantly within the PVA beads. High ammonia-assimilating isolates in the mixed culture containing viable lagoon microorganisms were identified as Pseudomonas spp. and member of Rhizobiaceae species by partial sequencing of the 16S ribosomal DNA.
More Related Videos
Related Concept Videos
Microbial Wastewater Treatment
Comparative Excretory Systems
Overview of Nitrogen Metabolism
The largest pool of nitrogen available in the terrestrial ecosystem is gaseous nitrogen (N2) from the air, but this nitrogen...
Inorganic Nitrogen Assimilation
Microbial Bioremediation of Hydrocarbons
Production of Alcohol

