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Updated: Jul 20, 2026
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Flow Cytometric Analysis of Biomarkers for Detecting Human Sperm Functional Defects
Published on: April 21, 2022
Direct nitric oxide measurement in human spermatozoa: flow cytometric analysis using the fluorescent probe,
Fanuel Lampiao1, Hans Strijdom, Stefan S du Plessis
1Department of Medical Physiology and Biochemistry, University of Stellenbosch, Tygerberg, South Africa.
International Journal of Andrology
|September 14, 2006
Abstract:
We have demonstrated that intracellular nitric oxide (NO) can be detected and measured directly in human spermatozoa by flow cytometry using the fluorescent probe 4,5-diaminofluorescein-2/diacetate (10 microM). This method can measure both exogenously added as well as endogenously generated intracellular NO.
