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Updated: Jul 20, 2026
14:55
Production and Purification of Non Replicative Canine Adenovirus Type 2 Derived Vectors
Published on: December 3, 2013
Foamy-virus-mediated gene transfer to canine repopulating cells
Hans-Peter Kiem1, James Allen, Grant Trobridge
1Clinical Division, Fred Hutchinson Cancer Research Center, 1100 Fairview Ave N D1-100, PO Box 19024, Seattle, WA 98109-1024, USA. hkiem@fhcrc.org
Blood
|September 14, 2006
Summary
Foamy virus vectors efficiently transduced canine hematopoietic stem cells, leading to multilineage engraftment. This shows promise for stem cell gene therapy, especially when short transduction times are needed.
Area of Science:
- Gene therapy
- Stem cell biology
- Retroviral vectors
Background:
- Foamy virus (FV) vectors are attractive for gene therapy due to stable transduction intermediates in quiescent cells.
- They efficiently transduce hematopoietic stem cells, making them suitable for stem cell gene therapy.
Purpose of the Study:
- To evaluate foamy virus vectors for transducing long-term hematopoietic repopulating cells in a canine model.
- To assess the efficiency and multilineage engraftment of FV vector-transduced cells.
Main Methods:
- Mobilized canine peripheral blood CD34+ cells were transduced with an EGFP-expressing FV vector.
- Transduction efficiency was analyzed using real-time PCR and Southern blot.
- Integration site analysis was performed to confirm polyclonal repopulation.
Main Results:
- Rapid neutrophil engraftment was observed in all dogs within 10 days.
- Transgene expression (EGFP) was detected in all blood cell lineages, indicating multilineage engraftment.
- Transduction rates were higher than previously achieved with lentiviral vectors.
Conclusions:
- FV vectors are effective for transducing hematopoietic stem cells in a large animal model.
- The results suggest FV vectors are highly promising for stem cell gene therapy, particularly for protocols requiring short transduction times.
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