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Related Experiment Video

Updated: Jul 20, 2026

From a Natural Product to Its Biosynthetic Gene Cluster: A Demonstration Using Polyketomycin from Streptomyces diastatochromogenes Tü6028

From a Natural Product to Its Biosynthetic Gene Cluster: A Demonstration Using Polyketomycin from Streptomyces diastatochromogenes Tü6028

Published on: January 13, 2017

Structural and mechanistic insights into polyketide macrolactonization from polyketide-based affinity labels.

John W Giraldes¹, David L Akey, Jeffrey D Kittendorf

¹Department of Medicinal Chemistry, University of Minnesota, 308 Harvard Street S.E., 8-101 Weaver-Densford Hall, Minneapolis, Minnesota 55455-0353, USA.

Nature Chemical Biology

|September 14, 2006

Summary

Researchers synthesized polyketide-based affinity labels to study polyketide synthases (PKSs). Crystal structures of these labels bound to thioesterase enzymes offer mechanistic insights into PKS function and engineering.

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From a Natural Product to Its Biosynthetic Gene Cluster: A Demonstration Using Polyketomycin from Streptomyces diastatochromogenes Tü6028

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Area of Science:

Biochemistry
Natural Product Biosynthesis
Structural Biology

Background:

Polyketides are natural products with significant clinical applications, including antibiotics and anticancer agents.
Polyketide synthases (PKSs) are large multienzyme complexes responsible for polyketide biosynthesis.
Understanding PKS mechanisms is crucial for drug discovery and combinatorial biosynthesis.

Purpose of the Study:

To chemically synthesize polyketide-based affinity labels.
To investigate the mechanism of thioesterase domains within PKSs.
To provide structural insights into substrate-enzyme interactions.

Main Methods:

Chemical synthesis of novel polyketide-based affinity labels.
Covalent modification of pikromycin thioesterase active site serine.
X-ray crystallography of enzyme-affinity label adducts.

Main Results:

Successful synthesis of polyketide-based affinity labels.
Obtained crystal structures of affinity label-thioesterase adducts.
Provided mechanistic insights into thioesterase activity within PKSs.

Conclusions:

Affinity labels are effective tools for studying individual PKS domains.
Structural data elucidates key steps in polyketide biosynthesis.
These findings facilitate rational engineering of PKSs for novel compound generation.