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Amylase-producing plasmacytoma cell lines, AD3 and FR4, with der(14)t(8;14) and dic(8)t(1;8) established from ascites
1Department of Clinical Research, Osaka University, Japan.
Abstract:
We established two human plasma cell lines, FR4 and AD3, from ascitic fluid in a patient with IgA k plasmacytoma (PC). Aberrant amylase production was found in this patient. Both AD3 and FR4 were free of Epstein-Barr virus, and both produced Ig A k in vitro. They produced amylase of the salivary type in vitro. This was confirmed by the demonstration of amylase mRNA comigrating with salivary gland mRNA. These cell lines commonly had unusual chromosomal abnormalities der(14)t(8;14) and dic(8)t(1;8). AD3 had additional chromosomal abnormalities compared with FR4. This suggests that AD3 is a subline of FR4. The oncogene c-myc is rearranged in most case of Burkitt's lymphoma with t(8;14). However, neither rearrangement nor amplification of the c-myc allele was detected in our PC lines. These lines expressed c-myc of 2.4 kb. There were no structural changes in the amylase genes of AD3 and FR4 detectable with Southern blotting analysis. As these lines were authentic PC lines, they would be useful for the future study of the relationship between the mechanism of oncogenesis and the rare tumor aberration, amylase production.
Insights
Two human plasma cell lines (PC) were established from a patient with IgA kappa plasmacytoma. These cell lines exhibit unusual chromosomal abnormalities and produce salivary-type amylase, offering insights into rare tumor aberrations.
Area of Science:
- Oncology
- Cell Biology
- Genetics
Background:
- Established two human plasma cell lines (PC), FR4 and AD3, from ascitic fluid of a patient diagnosed with IgA kappa plasmacytoma.
- Investigated aberrant amylase production, a rare tumor characteristic, in these established cell lines.
Observation:
- Both cell lines, FR4 and AD3, were confirmed to be Epstein-Barr virus-negative.
- Both cell lines demonstrated in vitro production of IgA kappa and salivary-type amylase, with corresponding amylase mRNA detected.
- Unusual chromosomal abnormalities, specifically der(14)t(8;14) and dic(8)t(1;8), were identified in both cell lines, with AD3 exhibiting additional abnormalities suggesting it is a subline of FR4.
Findings:
- Despite the common t(8;14) translocation associated with Burkitt's lymphoma, neither rearrangement nor amplification of the c-myc oncogene was detected in the PC lines.
- Southern blotting analysis revealed no structural changes in the amylase genes of either cell line.
- The cell lines expressed a 2.4 kb c-myc transcript.
Implications:
- These authentic PC cell lines provide a valuable model for studying the oncogenesis mechanisms underlying rare tumor aberrations, such as aberrant amylase production.
- Further research using these cell lines can elucidate the relationship between specific chromosomal abnormalities and the development of plasmacytoma with unique secretory functions.