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PHProteomicDB: a module for two-dimensional gel electrophoresis database creation on personal web sites
Pascal Pernet1, Arnaud Bruneel, Bruno Baudin
1Service de Biochimie A, Hopital Saint-Antoine, 75012 Paris, France. pascal.pernet@sat.aphp.fr
Genomics, Proteomics & Bioinformatics
|September 15, 2006
Summary
PHProteomicDB is a user-friendly module for proteomics researchers to share 2D electrophoresis gel data online. It requires minimal technical knowledge, enabling easy data entry and display on personal websites.
Area of Science:
- Proteomics
- Bioinformatics
- Web Technologies
Background:
- Sharing and managing two-dimensional electrophoresis (2DE) gel data is crucial for proteomics research.
- Existing methods for data dissemination can be technically demanding for researchers without specialized web development skills.
Purpose of the Study:
- To introduce PHProteomicDB, a novel PHP-based module designed to simplify the sharing of 2DE gel data.
- To provide proteomics researchers with an accessible tool for creating personal websites to showcase their experimental results.
Main Methods:
- PHProteomicDB is a PHP-written module that requires basic website management knowledge.
- It features a user-friendly administration interface for data entry and updates.
- The module dynamically generates web pages displaying gel characteristics, images, and spot identifications linked to protein databases.
Main Results:
- Enables researchers to easily upload and display 2DE gel data, including images and spot details.
- Facilitates the linking of identified gel spots to external protein databanks for further reference.
- Creates accessible web pages for sharing proteomics data without advanced technical expertise.
Conclusions:
- PHProteomicDB offers a straightforward solution for proteomics researchers to share 2DE gel data.
- The module lowers the technical barrier for data dissemination, promoting wider accessibility and collaboration.
- It empowers researchers to manage and present their proteomics findings effectively on personal websites.
Related Concept Videos
Two-dimensional Gel Electrophoresis
Two-dimensional gel electrophoresis is a high-resolution protein separation method first introduced by O' Farrell and Klose in 1975. This method involves protein separation by two dimensions, mass and charge, making it more accurate than one-dimensional gel electrophoresis.
The first dimension separation uses the isoelectric focusing or IEF technique performed on immobilized pH gradient (IPG) strips that separate proteins according to their isoelectric points.
Biological samples, such as cells...
The first dimension separation uses the isoelectric focusing or IEF technique performed on immobilized pH gradient (IPG) strips that separate proteins according to their isoelectric points.
Biological samples, such as cells...
SDS-PAGE
Gel electrophoresis is a method that separates biological macromolecules like nucleic acids or proteins by forcing them to pass through a gel matrix under an electric field.
A variation of gel electrophoresis, termed polyacrylamide gel electrophoresis (PAGE), is commonly used for separating proteins according to their molecular size by passing them through a polyacrylamide gel. Because of the varying charges associated with amino acid side chains, PAGE can be used to separate intact proteins...
A variation of gel electrophoresis, termed polyacrylamide gel electrophoresis (PAGE), is commonly used for separating proteins according to their molecular size by passing them through a polyacrylamide gel. Because of the varying charges associated with amino acid side chains, PAGE can be used to separate intact proteins...

