Comparison of biochemical parameters of Muscovy drake semen diluted and stored at 4 degrees C in three buffers

A comparison study of biochemical parameters of semen from Muscovy drakes diluted and stored at 4 degrees C in three buffers--IMV-buffer (France), HIA-1 and AU (Bulgaria) was carried out. The ejaculates were collected twice a week from ten 1-year-old Muscovy drakes using laying Muscovy females as teaser. Semen was diluted immediately, respectively, with IMV-buffer, HIA-1 and AU, and cold-stored (4 degrees C) for 1, 3 and 6h. The intensities of oxygen uptake at the third hour in semen diluted, respectively, with IMV-buffer (200+/-1.6 nAO/10(9) spermcellsmin), with HIA (224+/-44 nAO/10(9) spermcellsmin) and with AU (238+/-48 nAO/10(9) spermcellsmin) were highly significant in comparison with neat semen (75+/-0.7 nAO/10(9)spermcellsmin). The observed intensity of fructolysis was highest when using AU, followed by HIA-1 and IMV-buffer. During the first hour of storage the level of pyruvic acid was significantly lower in semen diluted with Bulgarian extenders, and this stability for AU referred to the entire period. For lactic acid, the differences were not statistical significant. Our investigations do not show significant differences concerning the dynamics of inorganic phosphate and total lipids after dilution with all tested extenders. On the contrary, high increase of cholesterol efflux from spermatozoa to seminal plasma-diluents were obtained after 6 h of storage. All extenders, IMV-buffer (France), HIA-1 and AU (Bulgaria) for diluting and short time storage of semen from Muscovy drakes at 4 degrees C maintain the necessary comfort of energy metabolism of the spermatozoa.