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Related Experiment Videos

Simultaneous detection of creatine and creatinine using a sequential injection analysis/biosensor system.

Raluca-Ioana Stefan-van Staden1, Rahel Girmai Bokretsion, Jacobus F van Staden

  • 1Faculty of Chemistry, University of Bucharest, Bucharest, Romania. iustinavanstaden@yahoo.com

Preparative Biochemistry & Biotechnology
|September 15, 2006
PubMed
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This study presents a novel sequential injection analysis (SIA) system using carbon paste biosensors for rapid creatine and creatinine detection. The system achieves high sensitivity and reliability for simultaneous analysis in pharmaceutical and serum samples.

Area of Science:

  • Analytical Chemistry
  • Biosensor Technology
  • Enzyme Kinetics

Background:

  • Accurate determination of creatine and creatinine is crucial for diagnosing metabolic disorders and assessing kidney function.
  • Existing analytical methods can be time-consuming or require complex sample preparation.
  • Development of rapid, sensitive, and reliable biosensing platforms is needed.

Purpose of the Study:

  • To develop and validate a sequential injection analysis (SIA) system for the simultaneous determination of creatine and creatinine.
  • To utilize carbon paste based biosensors integrated with a multi-enzyme system for enhanced detection.
  • To assess the system's performance in terms of sensitivity, detection limits, and sample throughput.

Main Methods:

  • Carbon paste biosensors were fabricated and modified with a multi-enzyme sequence: creatininase (CA), creatinase (CI), and sarcosine oxidase (SO).

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  • Amperometric detection of hydrogen peroxide produced during enzymatic reactions was employed.
  • The biosensors were integrated into a sequential injection system for automated sample processing.

    • Main Results:

    • The SIA system demonstrated linear concentration ranges from pmol/L to nmol/L for both creatine and creatinine.
    • Very low limits of detection were achieved, indicating high sensitivity.
    • The system provided reliable on-line simultaneous detection with a sample throughput of 34 samples per hour.
    • Excellent precision was observed with relative standard deviation (RSD) values better than 0.16% (n=10).

    Conclusions:

    • The developed carbon paste biosensor-based SIA system offers a sensitive, rapid, and reliable method for simultaneous creatine and creatinine determination.
    • This approach is suitable for quality control of pharmaceutical products and clinical analysis of serum samples.
    • The high sample throughput and low detection limits make it a valuable tool for routine analysis.