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Related Experiment Videos

Chromogenic assay for equine plasminogen.

E G Welles1, K W Prasse, A Duncan

  • 1Department of Pathology, College of Veterinary Medicine, University of Georgia, Athens 30602.

American Journal of Veterinary Research
|July 1, 1990
PubMed
Summary

A new assay effectively measures equine plasminogen function. Acidification inactivates alpha-2-antiplasmin, enabling accurate urokinase-activated plasminogen measurement in horses.

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Area of Science:

  • Veterinary Hematology
  • Biochemical Assays
  • Fibrinolysis Research

Background:

  • Plasminogen is crucial for fibrinolysis.
  • Accurate measurement of equine plasminogen is vital for veterinary diagnostics.
  • Existing assays may be limited by inhibitors like alpha-2-antiplasmin.

Purpose of the Study:

  • To establish a reliable functional assay for equine plasminogen.
  • To investigate the role of plasma acidification in assay performance.
  • To determine optimal conditions for equine plasminogen activation.

Main Methods:

  • Developed a functional assay using urokinase activator and a chromogenic substrate.
  • Employed a computer-assisted centrifugal analyzer for precise measurements.
  • Utilized acidified/neutralized equine plasma to investigate inhibitor effects.

Main Results:

  • The assay demonstrated good precision (intra-assay CV 4.1%, inter-assay CV 5.6%).
  • Equine plasminogen remained stable for 1 week at 4°C and 5 months at -70°C.
  • Acidification inactivated alpha-2-antiplasmin, allowing urokinase-mediated plasminogen activation.

Conclusions:

  • A robust functional assay for equine plasminogen has been established.
  • Plasma acidification is a key step for accurate measurement by inactivating alpha-2-antiplasmin.
  • The assay provides a valuable tool for equine hemostasis research and diagnostics.

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