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Related Concept Videos

Immunofluorescence Microscopy01:12

Immunofluorescence Microscopy

A fluorescence microscope uses fluorescent chromophores called fluorochromes, which can absorb energy from a light source and then emit this energy as visible light. Fluorochromes include naturally fluorescent substances (such as chlorophylls) and fluorescent stains that are added to the specimen to create contrast. Dyes such as Texas red and FITC are examples of fluorochromes. Other examples include the nucleic acid dyes 4’,6’-diamidino-2-phenylindole (DAPI), and acridine orange.
The...

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Technique of Conjunctival Biopsy and Direct Immunofluorescence for Diagnosing Mucous Membrane Pemphigoid
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Dark immunofluorescence: correlation with serum immunoglobulin abnormalities.

J List1, M S Buckland, B Thobhani

  • 1Department of Haematology Immunology Section, Colchester General Hospital, Turner Road, Colchester, Essex CO4 5JL, United Kingdom. jenny.list@essexrivers.nhs.uk

Clinical and Vaccine Immunology : CVI
|September 15, 2006
PubMed
Summary

Low fluorescence in occasional serum samples may indicate immunoglobulin abnormalities. A review found 71% of such patients had abnormal immunoglobulins, suggesting this finding warrants clinical attention.

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Area of Science:

  • Clinical Chemistry
  • Immunology

Background:

  • Indirect immunofluorescence is a common diagnostic technique.
  • Serum immunoglobulin levels are crucial indicators of immune status.

Purpose of the Study:

  • To investigate the significance of decreased fluorescence in indirect immunofluorescence assays.
  • To determine the association between reduced fluorescence and serum immunoglobulin abnormalities.

Main Methods:

  • Retrospective review of patient serum samples with observed low indirect immunofluorescence.
  • Analysis of serum immunoglobulin levels in affected patients and controls.

Main Results:

  • Less than 0.5% of serum samples showed reduced fluorescence compared to negative controls.
  • A high percentage (71%) of patients with reduced fluorescence exhibited immunoglobulin abnormalities.
  • Control groups showed significantly lower rates of abnormalities (22%).

Conclusions:

  • Reduced indirect immunofluorescence in serum samples may be a marker for underlying immunoglobulin abnormalities.
  • Clinicians should be alerted to this potential association and consider further immunoglobulin testing.