Related Experiment Video
Updated: Jul 20, 2026
Microfluidics in Assessing Platelet Function
Published on: November 8, 2024
Comparison of changes in circulating platelet-derived microparticles and platelet surface P-selectin expression after
Teruo Inoue1, Yutaka Hikichi, Toshihumi Morooka
1Faculty of Medicine, Department of Cardiovascular and Renal Medicine, Saga University, Saga, Japan. inouete@med.saga-u.ac.jp
Abstract:
Platelet-derived microparticles (PDMPs) are released from activated platelets and may participate in the inflammatory process in response to vessel wall injury. This study was designed to compare the clinical significance of circulating PDMPs with that of P-selectin on the platelet membrane surface. In 20 patients with stable angina undergoing coronary stent implantation, circulating PDMPs were serially measured by enzyme-linked immunosorbent assay, and P-selectin expression on the surface of platelets was simultaneously analyzed by flow cytometry. PDMPs increased 24-48 h after coronary stenting in the coronary sinus (8.7 +/- 8.9 to 31.8 +/- 19.8 U/ml, P < 0.001) with a maximum at 48 h. In contrast, the mean channel fluorescence intensity for P-selectin increased 15 min after coronary stenting in the coronary sinus (19.5 +/- 5.6 to 25.2 +/- 7.5, P < 0.01) and remained elevated for 48 h; the changes were less striking in peripheral blood. The relative increase in PDMPs was not correlated with the increase in P-selectin expression at 15 min or 24 h after coronary stenting, but was correlated at 48 h (R = 0.48, P < 0.05). Both circulating PDMPs and P-selectin expression were enhanced in association with stent-induced platelet activation; however, the time course of changes in these two platelet activation markers was different. Therefore, the clinical relevance of circulating PDMPs may differ from that of P-selectin expression on the platelet membrane surface.
Insights
Platelet-derived microparticles (PDMPs) and P-selectin both indicate platelet activation after coronary stenting, but their release patterns differ. PDMPs rise later, suggesting distinct clinical relevance compared to P-selectin.
Area of Science:
- Cardiovascular Biology
- Platelet Physiology
- Biomarker Research
Background:
- Platelet-derived microparticles (PDMPs) are implicated in inflammation following vessel wall injury.
- P-selectin is a surface marker of platelet activation.
Purpose of the Study:
- To compare the clinical significance of circulating PDMPs with P-selectin expression on platelet surfaces.
- To investigate platelet activation markers after coronary stent implantation.
Main Methods:
- Enzyme-linked immunosorbent assay (ELISA) for PDMPs.
- Flow cytometry for P-selectin expression.
- Serial measurements in coronary sinus and peripheral blood of 20 stable angina patients undergoing stenting.
Main Results:
- PDMPs significantly increased 24-48 hours post-stenting, peaking at 48 hours.
- P-selectin expression increased rapidly at 15 minutes and remained elevated for 48 hours.
- PDMP increase correlated with P-selectin at 48 hours, but not earlier.
Conclusions:
- Both PDMPs and P-selectin indicate stent-induced platelet activation.
- The distinct temporal profiles suggest differing clinical relevance for PDMPs and P-selectin.
- Further research is needed to elucidate the specific roles of PDMPs in cardiovascular events.
