Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been developed.
Confocal Fluorescence Microscopy01:16

Confocal Fluorescence Microscopy

Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...
Imaging Biological Samples with Optical Microscopy01:18

Imaging Biological Samples with Optical Microscopy

Optical microscopy uses optic principles to provide detailed images of samples. Antonie van Leeuwenhoek designed the first compound optical microscope in the 17th century to visualize blood cells, bacteria, and yeast cells. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes with enhanced magnification and resolution.
In optical microscopy, the specimen to be viewed is placed on a glass slide and clipped on the stage...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Molecular heterogeneity of HPV-associated cancers and strategies to overcome treatment resistance.

Cancer heterogeneity and plasticity·2026
Same author

Real-space observation of flat-band ultrastrong coupling between optical phonons and surface plasmon polaritons.

Nature materials·2025
Same author

Combining quantum cascade lasers and plasmonic metasurfaces to monitor <i>de novo</i> lipogenesis with vibrational contrast microscopy.

Nanophotonics (Berlin, Germany)·2025
Same author

Tailoring Phonon Polaritons in hBN with the Plasmonic Phase-Change Material In<sub>3</sub>SbTe<sub>2</sub>.

Nano letters·2025
Same author

Two-dimensional talc as a natural abundant ultra-broadband hyperbolic material.

Nanoscale·2025
Same author

Immune and Biological Changes during Treatment in Patients with Nonsegmental Vitiligo and their Relation to Repigmentation.

The Journal of investigative dermatology·2025

Related Experiment Video

Updated: Jul 20, 2026

Demonstration of a Hyperlens-integrated Microscope and Super-resolution Imaging
10:01

Demonstration of a Hyperlens-integrated Microscope and Super-resolution Imaging

Published on: September 8, 2017

Near-field microscopy through a SiC superlens.

Thomas Taubner1, Dmitriy Korobkin, Yaroslav Urzhumov

  • 1Nano-Photonics Group, Max-Planck-Institut für Biochemie, Am Klopferspitz 18, D-82152 Martinsried, Germany.

Science (New York, N.Y.)
|September 16, 2006
PubMed
Summary

This study introduces a new optical imaging system that combines superlensing and scanning near-field optical microscopy (SNOM). The technique achieves subwavelength resolution for subsurface objects without requiring close probe proximity.

More Related Videos

Super-Resolution Microscopy of the Synaptonemal Complex Within the Caenorhabditis elegans Germline
09:14

Super-Resolution Microscopy of the Synaptonemal Complex Within the Caenorhabditis elegans Germline

Published on: September 13, 2022

High-Throughput Total Internal Reflection Fluorescence and Direct Stochastic Optical Reconstruction Microscopy Using a Photonic Chip
14:09

High-Throughput Total Internal Reflection Fluorescence and Direct Stochastic Optical Reconstruction Microscopy Using a Photonic Chip

Published on: November 16, 2019

Related Experiment Videos

Last Updated: Jul 20, 2026

Demonstration of a Hyperlens-integrated Microscope and Super-resolution Imaging
10:01

Demonstration of a Hyperlens-integrated Microscope and Super-resolution Imaging

Published on: September 8, 2017

Super-Resolution Microscopy of the Synaptonemal Complex Within the Caenorhabditis elegans Germline
09:14

Super-Resolution Microscopy of the Synaptonemal Complex Within the Caenorhabditis elegans Germline

Published on: September 13, 2022

High-Throughput Total Internal Reflection Fluorescence and Direct Stochastic Optical Reconstruction Microscopy Using a Photonic Chip
14:09

High-Throughput Total Internal Reflection Fluorescence and Direct Stochastic Optical Reconstruction Microscopy Using a Photonic Chip

Published on: November 16, 2019

Area of Science:

  • Optics
  • Microscopy
  • Nanotechnology

Background:

  • Classical microscopy is limited by the diffraction limit of light, restricting spatial resolution.
  • Superlensing offers subwavelength resolution but has limitations.
  • Scanning near-field optical microscopy (SNOM) provides high resolution but requires close proximity to the sample.

Purpose of the Study:

  • To develop a novel imaging system combining superlensing and SNOM.
  • To achieve subwavelength lateral resolution for subsurface objects.
  • To overcome the proximity requirement of traditional SNOM.

Main Methods:

  • Integration of superlensing principles with SNOM.
  • Development of a novel optical near-field microscopy system.
  • Imaging of subsurface objects at subwavelength scales.

Main Results:

  • Demonstration of a novel imaging system.
  • Achieved subwavelength-scale lateral resolution for subsurface objects.
  • Eliminated the need for close proximity between the object and the near-field probe.

Conclusions:

  • The developed system offers a new approach for high-resolution subsurface imaging.
  • This technique overcomes key limitations of existing microscopy methods.
  • Enables optical near-field microscopy with enhanced capabilities.