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[Antibodies against hyaline cartilage. Antigen isolation and their characterization]
1Oddelenia pre výskum histochémie a ultrastruktúrnej patológie Centra klinických oddelení VULB v Bratislave.
Bratislavske Lekarske Listy
|June 1, 1990
Abstract:
Extracts from human hyaline cartilage were analyzed quantitatively. The extracts were obtained by the action of salts, enzymatic processing and chromatography on Sephadex G-200 columns. The obtained fractions were characterized by thin layer chromatographic methods, electrophoretically, and immunochemically by means of polyclonal antibodies to individual components. Qualitative analysis of amino acids and electrophoretic separation showed that the 1st fraction contained mainly proteins and glycosaminoglycans after separation on Sephadex G-200, while the 2nd fraction exhibited mainly amino acids characteristic for collagen.