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An ultrastructural study of the guinea pig tectorial membrane 'type A' protofibril
T Arima1, D J Lim, H Kawaguchi
1Department of Otolaryngology, Faculty of Medicine, Kyushu University, Fukuoka, Japan.
Hearing Research
|July 1, 1990
Abstract:
Fine structural features of the 'type A' protofibrils in the guinea pig tectorial membrane were examined using negative staining and deep-etching techniques. Negative-stained samples of fragmented tectorial membrane were composed of several fine filamentous subunits showing the clear banding pattern of the type A protofibrils. Deep-etched replicas of the EGTA (ethylene glycol bis-N,N,N',N'-tetraacetic acid)-treated samples showed fine surface structure consisting of several linear arrays of filamentous elements on the extracellular fibrils, which is interpreted to be type A protofibrils.