Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Capillary separations enabling tissue proteomics-based biomarker discovery.

Tong Guo1, Cheng S Lee, Weijie Wang

  • 1Department of Chemistry and Biochemistry, University of Maryland, College Park, MD, USA.

Electrophoresis
|September 16, 2006
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Clinical and neuroimaging correlates of motoric cognitive risk syndrome in cerebral small vessel disease: a cross-sectional study.

Frontiers in aging neuroscience·2026
Same author

Rearing systems shape the successional dynamics of the gut microbiota, resistome, and mobilome in Lueyang Black-boned chickens.

Poultry science·2026
Same author

Nanoarmored Probiotic for Inflammatory Bowel Disease Bacteriotherapy via a Dual-Targeting Host-Microbiota Reprogramming.

ACS nano·2026
Same author

SlCOMT1-dependent melatonin biosynthesis is required for auxin-mediated alleviation of cadmium toxicity in tomato.

Journal of plant physiology·2026
Same author

Phase I, Randomized, Double-Blind, Placebo-Controlled Trial Investigating the Safety, Tolerability, and Pharmacokinetics of BS1801 in Healthy Chinese Adult Subjects.

Drug design, development and therapy·2026
Same author

Diagnostic and short-term prognosis value of serum ox-LDL/LOX-1 and GDF-15 in elderly patients with acute coronary syndrome.

Frontiers in molecular biosciences·2026
Same journal

Kinship Inferences for Second-Degree Relatives With a Combination of STRs and Microhaplotypes.

Electrophoresis·2026
Same journal

Optimisation of Electrokinetic Extraction System: Colourimetric Determination of Copper (II) in Sand Using Polymer Inclusion Membrane.

Electrophoresis·2026
Same journal

Novel Phloroglucinol Derivatives as Neuraminidase Inhibitors Identified From Humulus lupulus L. Extract by At-Line Nanofractionation Platform.

Electrophoresis·2026
Same journal

Protein-Based High-Performance Liquid Chromatography and Cyclodextrin-Capillary Electrokinetic Chromatography for the Chiral Separation of Azoles.

Electrophoresis·2026
Same journal

Dynamics of Heparin Translocations Through Solid-State Nanopores.

Electrophoresis·2026
Same journal

Production of Protein Hydrolysates and Bioactive Peptides From Lablab purpureus and Macrotyloma uniflorum via Optimized Extraction and Proteolysis Protocols.

Electrophoresis·2026
See all related articles

Large-scale proteome studies using advanced microdissection and separation techniques enable comprehensive protein analysis in tissues. This facilitates protein biomarker discovery for diseases like cancer, improving diagnostic and therapeutic strategies.

Area of Science:

  • Proteomics
  • Biomarker Discovery
  • Cancer Research

Background:

  • Large-scale proteome studies are crucial for understanding human diseases, particularly cancer.
  • Interrogating archived tissue collections (fresh frozen, FFPE) requires advanced techniques.
  • Protein biomarker investigations are vital for disease diagnosis and treatment.

Purpose of the Study:

  • To review recent advances in microdissection and proteome platforms for tissue analysis.
  • To highlight the role of capillary separations and multidimensional separations in proteome analysis.
  • To explore opportunities for biomarker discovery using enriched cell populations from tissues.

Main Methods:

  • Microdissection techniques for isolating homogeneous cell subpopulations.

Related Experiment Videos

  • Proteome platforms for comprehensive protein profile analysis.
  • Capillary separations (e.g., CIEF-based multidimensional separations) for high-resolution proteome analysis.

    • Main Results:

    • Recent advances enable procurement of specific cell populations from tissue specimens.
    • High-resolution separation techniques allow for detailed proteome analysis from minute samples.
    • Integrated tissue proteome efforts are advancing biomarker discovery.

    Conclusions:

    • Technological advancements in microdissection and proteome analysis offer powerful tools for disease research.
    • These tools enhance the understanding of cellular machinery in diseases like cancer.
    • Opportunities for protein biomarker discovery are significantly expanded.