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[Exclusion chromatography for the separation of cryoprotective agents from freeze-preserved blood cells]
1Institut für Transfusiologie und Transplantologie, Humboldt-Universität, Berlin.
Folia Haematologica (Leipzig, Germany : 1928)
|January 1, 1990
Abstract:
A method for separating low molecular cryoprotectiva from freeze-conserved erythrocyte- and thrombocyte-concentrates by exclusion chromatography has been described. A new vesicular packing material has been used. Only 25 to 30 minutes are necessary in order to separate glycerol respectively dimethylsulphoxide (DMSO) completely from the cells. 86.5% of the erythrocytes and 75.4% of the thrombocytes were recovered after the separation process.