Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

G-protein Coupled Receptors01:21

G-protein Coupled Receptors

G-protein coupled receptors are ligand binding receptors that indirectly affect changes in the cell. The actual receptor is a single polypeptide that transverses the cell membrane seven times creating intracellular and extracellular loops. The extracellular loops create a ligand specific pocket which binds to neurotransmitters or hormones. The intracellular loops holds onto the G-protein.
Long-term Depression01:03

Long-term Depression

Long-term depression, or LTD, is one of the ways by which synaptic plasticity—changes in the strength of chemical synapses—can occur in the brain. LTD is the process of synaptic weakening that occurs over time between pre and postsynaptic neuronal connections. The synaptic weakening of LTD works in opposition to synaptic strengthening by long-term potentiation (LTP) and together are the main mechanisms that underlie learning and memory.
Calcium Ion Concentration Mechanism
If over time, all...
Long-term Depression01:05

Long-term Depression

Long-term depression, or LTD, is one of the ways by which synaptic plasticity—changes in the strength of chemical synapses—can occur in the brain. LTD is the process of synaptic weakening that occurs over time between pre and postsynaptic neuronal connections. The synaptic weakening of LTD works in opposition to synaptic strengthening by long-term potentiation (LTP) and together are the main mechanisms that underlie learning and memory.

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

All-electron quasiparticle self-consistent GW for molecules and periodic systems within the numerical atomic orbital framework.

The Journal of chemical physics·2026
Same author

Low-Scaling GW Calculations of Quasi-Particle Energies for Extended Systems within the Numerical Atomic Orbital Framework.

Journal of chemical theory and computation·2026
Same author

Real-Time Time-Dependent Density Functional Theory Simulations with Range-Separated Hybrid Functionals for Periodic Systems.

Journal of chemical theory and computation·2026
Same author

First-Principles Approach to Electron-Vibration Interaction in Molecules from an Atomic Orbital Basis: The Allen-Heine-Cardona Theory and Beyond.

The journal of physical chemistry letters·2026
Same author

Intranasal 5-MeO-DMT Concomitant with SSRI for Treatment-Resistant Depression: A Proof-of-Concept Trial.

CNS drugs·2026
Same author

ABACUS: An electronic structure analysis package for the AI era.

The Journal of chemical physics·2025

Related Experiment Video

Updated: Jul 20, 2026

Lipidomics and Transcriptomics in Neurological Diseases
09:58

Lipidomics and Transcriptomics in Neurological Diseases

Published on: March 18, 2022

Cyclic AMP response element-binding protein in post-mortem brain of teenage suicide victims: specific decrease in the

Ghanshyam N Pandey1, Yogesh Dwivedi, Xinguo Ren

  • 1The Psychiatric Institute, Department of Psychiatry, University of Illinois at Chicago, Chicago, IL 60612, USA. Gnpandey@psych.uic.edu

The International Journal of Neuropsychopharmacology
|September 19, 2006
PubMed
Summary

In teenage suicide victims, cyclic AMP response element-binding protein (CREB) levels and activity were significantly reduced in the prefrontal cortex. These findings link CREB dysfunction to signaling pathway abnormalities in suicide pathophysiology.

More Related Videos

Stereotactic Atlas-Guided Laser Capture Microdissection of Brain Regions Affected by Traumatic Injury
09:29

Stereotactic Atlas-Guided Laser Capture Microdissection of Brain Regions Affected by Traumatic Injury

Published on: September 11, 2017

A Plate-Based Assay for the Measurement of Endogenous Monoamine Release in Acute Brain Slices
07:56

A Plate-Based Assay for the Measurement of Endogenous Monoamine Release in Acute Brain Slices

Published on: August 11, 2021

Related Experiment Videos

Last Updated: Jul 20, 2026

Lipidomics and Transcriptomics in Neurological Diseases
09:58

Lipidomics and Transcriptomics in Neurological Diseases

Published on: March 18, 2022

Stereotactic Atlas-Guided Laser Capture Microdissection of Brain Regions Affected by Traumatic Injury
09:29

Stereotactic Atlas-Guided Laser Capture Microdissection of Brain Regions Affected by Traumatic Injury

Published on: September 11, 2017

A Plate-Based Assay for the Measurement of Endogenous Monoamine Release in Acute Brain Slices
07:56

A Plate-Based Assay for the Measurement of Endogenous Monoamine Release in Acute Brain Slices

Published on: August 11, 2021

Area of Science:

  • Neuroscience
  • Molecular Biology
  • Psychiatry

Background:

  • Post-mortem studies reveal abnormalities in adenylyl cyclase (AC) and phosphoinositide (PI) signaling in suicide victims.
  • Cyclic AMP response element-binding protein (CREB) is a crucial transcription factor activated by protein kinase A (PKA) and protein kinase C (PKC), suggesting convergence of AC and PI pathways at CREB.
  • CREB regulates genes implicated in the pathophysiology of depression and suicide.

Purpose of the Study:

  • To investigate whether observed PKA and PKC abnormalities in teenage suicide victims are associated with altered CREB function.
  • To examine CRE-DNA binding, protein expression, and mRNA expression of CREB in the prefrontal cortex (PFC) and hippocampus of teenage suicide victims.

Main Methods:

  • Gel shift assay for CRE-DNA binding.
  • Western blot technique for CREB protein expression.
  • Quantitative reverse transcriptase-polymerase chain reaction (RT-PCR) for CREB mRNA expression.
  • Analysis of PFC and hippocampus from 17 teenage suicide victims and 17 matched controls.

Main Results:

  • Significantly decreased CRE-DNA binding and protein expression of CREB in the PFC of teenage suicide victims compared to controls.
  • Significantly decreased mRNA expression of CREB in the PFC of teenage suicide victims.
  • No significant differences in CRE-DNA binding, protein, or mRNA expression of CREB in the hippocampus.

Conclusions:

  • Abnormalities in PKA and PKC observed in teenage suicide victims are associated with reduced CREB function in the PFC.
  • This CREB dysfunction may lead to alterations in the expression of critical neuronal genes, contributing to suicide pathophysiology.
  • Findings support the role of signal transduction abnormalities in suicide.