Effects of raclopride on aggression and stress in diversely selected chicken lines

Genetic selection for chickens of high (HGPS) and low (LGPS) group productivity and survivability, resulted in two distinct genetic lines characterized by differences in cannibalism, flightiness, and immunocompetence. Additionally, birds exhibited differences in behaviour and social stress coping strategy. HGPS birds have a superior stress coping strategy compared with birds of LGPS or Dekalb XL (DXL), a commercial strain. Line differences in stress response and behaviour could be due to selection-induced differences in expression of the dopaminergic system. The dopamine (D2) receptor, an integral part of the dopaminergic system, was hypothesized to be a key contributory factor of the stress response. We tested this hypothesis by injecting either a D2 antagonist (raclopride) or saline in the dominant individual in pair-housed birds for 10 days and examining stress coping ability. Results showed that dominant birds of all strains showed a reduced frequency of aggressive pecks on subordinates following raclopride injection. In contrast, subordinates paired with raclopride-injected birds increased pecking frequency. Two days after stopping injections, LGPS and DXL birds returned to pre-injection levels of aggressive threats, while HGPS birds maintained depressed frequency of threats. Strain differences in aggressive responsiveness coincided with increased epinephrine levels in raclopride treated LGPS birds relative to control LGPS birds, but not by HGPS and DXL birds. Our findings suggest a functional linkage between the genetic basis of stress coping ability and the dopamine regulation of aggressive responsiveness. The data further indicate that the sympathetic-adreno-medullary axis is directly involved in regulating both stress coping strategy and aggressiveness.