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Updated: Jul 20, 2026

Sample Preparation and Relative Quantitation using Reductive Methylation of Amines for Peptidomics Studies
Published on: November 4, 2021
Synthesis of D-labeled naphthyliodoacetamide and application to quantitative peptide analysis by isotope differential
Satomi Niwayama1, Sadamu Kurono, Hanjoung Cho
1Department of Chemistry and Biochemistry, Texas Tech University, Box 41061, Lubbock, TX 79409-1061, USA. satomi.niwayama@ttu.edu <satomi.niwayama@ttu.edu>
Abstract:
D-labeled and -unlabeled N-beta-naphthyliodoacetamides have been synthesized for specific modification of the sulfhydryl groups of cysteine residues in proteins or peptides, and have been applied to quantitative analysis of several peptides. A combination of these reagents, coupled with mass spectrometry, is anticipated to serve as a useful tool for quantitative analysis of peptides and hence proteins.
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