Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Quantification and thickness correction of EFTEM phosphorus maps.

M A Aronova1, Y C Kim, G Zhang

  • 1Division of Bioengineering and Physical Science, ORS, National Institutes of Health, Bethesda, MD 20892, USA.

Ultramicroscopy
|September 19, 2006
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

[Effects of radiofrequency catheter ablation in coagulation system].

Zhonghua nei ke za zhi·2002
Same author

Changes of serum selenium in pregnant women with gestational diabetes mellitus.

Biological trace element research·2002
Same author

[Exploration of weight-reducing effect of Kintop in obese rats and its mechanism].

Zhongguo Zhong xi yi jie he za zhi Zhongguo Zhongxiyi jiehe zazhi = Chinese journal of integrated traditional and Western medicine·2002
Same author

[Exploration on cause, pathogenesis and treatment of diabetic pedopathy].

Zhongguo Zhong xi yi jie he za zhi Zhongguo Zhongxiyi jiehe zazhi = Chinese journal of integrated traditional and Western medicine·2002
Same author

[Effect of kintop on gastrointestinal movement and its significance].

Zhongguo Zhong xi yi jie he za zhi Zhongguo Zhongxiyi jiehe zazhi = Chinese journal of integrated traditional and Western medicine·2002
Same author

[Experimental study on effect of bushen yijing recipe in delaying senility of bone and brain of aged male rats].

Zhongguo Zhong xi yi jie he za zhi Zhongguo Zhongxiyi jiehe zazhi = Chinese journal of integrated traditional and Western medicine·2002

This study introduces a new method for correcting scattering effects in elemental maps using energy filtering transmission electron microscopy (EFTEM). This technique improves the accuracy of phosphorus mapping in biological samples, even in thicker specimens.

Area of Science:

  • Materials Science
  • Microscopy Techniques
  • Analytical Chemistry

Background:

  • Inelastic scattering in electron energy loss spectroscopy (EELS) complicates elemental mapping.
  • Accurate elemental quantification in biological samples is crucial for research.

Purpose of the Study:

  • To develop and demonstrate a method for correcting plural inelastic scattering effects in EFTEM elemental maps.
  • To enable accurate mapping of low-concentration elements like phosphorus in biological specimens.

Main Methods:

  • Utilizing two energy windows (above and below a core edge) in EELS.
  • Generating standard curves of intensity ratios (k0) versus relative specimen thickness (t/lambda).
  • Acquiring zero-loss and unfiltered images to determine thickness and k0-ratio per pixel.

Related Experiment Videos

Main Results:

  • A novel method for correcting scattering effects in EFTEM elemental maps was successfully demonstrated.
  • The technique allows for accurate phosphorus mapping in biological samples, even with low concentrations.
  • Quantification of elemental maps in terms of atoms per pixel was achieved, with calculated errors.

Conclusions:

  • The developed two-window method significantly improves the accuracy of elemental mapping by correcting for thickness-dependent scattering.
  • This approach enables reliable phosphorus mapping in considerably thicker biological specimens than previously possible.
  • The method enhances the utility of EFTEM for quantitative elemental analysis in materials science and biology.