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Updated: Jul 20, 2026
09:01
Mutagenesis and Functional Selection Protocols for Directed Evolution of Proteins in E. coli
Published on: March 16, 2011
Role of DNA polymerase IV in Escherichia coli SOS mutator activity
Wojciech Kuban1, Magdalena Banach-Orlowska, Roel M Schaaper
1Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Pawinskiego 5A, 02 106 Warsaw, Poland.
Journal of Bacteriology
|September 19, 2006
Abstract:
Constitutive expression of the SOS regulon in Escherichia coli recA730 strains leads to a mutator phenotype (SOS mutator) that is dependent on DNA polymerase V (umuDC gene product). Here we show that a significant fraction of this effect also requires DNA polymerase IV (dinB gene product).
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