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Related Experiment Videos

Heme utilization in Campylobacter jejuni.

Kristian A Ridley1, Jonathan D Rock, Ying Li

  • 1Department of Genetics, University of Leicester, Adrian Building, University Road, Leicester LE1 7RH, United Kingdom.

Journal of Bacteriology
|September 19, 2006
PubMed
Summary
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Campylobacter jejuni utilizes heme and hemoglobin for iron via the ChuABCD transport system and Cj1613c heme oxygenase. Further inner membrane transport systems likely exist for heme uptake.

Area of Science:

  • Microbiology
  • Molecular Biology
  • Bacterial Pathogenesis

Background:

  • Heme and hemoglobin are crucial iron sources for many bacteria, including Campylobacter jejuni.
  • Iron acquisition mechanisms are vital for bacterial survival and virulence.
  • The specific pathways for heme uptake in C. jejuni were not fully elucidated.

Purpose of the Study:

  • To identify and characterize the genes involved in heme and hemoglobin utilization in Campylobacter jejuni.
  • To investigate the regulation of these genes by iron and the Fur protein.
  • To confirm the function of the putative heme oxygenase Cj1613c.

Main Methods:

  • Genetic mutation and complementation studies to assess gene function.
  • Genotyping of clinical isolates to determine gene conservation.

Related Experiment Videos

  • Analysis of promoter activity using reporter assays.
  • Gel retardation assays to study Fur protein binding.
  • Spectroscopic analysis to determine heme oxygenase activity.

    • Main Results:

    • The chuABCD genes and Cj1613c are essential for growth on hemin or hemoglobin as the sole iron source.
    • Mutations in chuB, -C, or -D only partially affected growth, suggesting additional uptake systems.
    • Cj1613c is highly conserved in clinical isolates.
    • The chuABCD and Cj1613c genes are independently iron-repressed and regulated by Fur.
    • Cj1613c functions as a heme oxygenase, degrading heme.

    Conclusions:

    • The ChuABCD transporter and Cj1613c heme oxygenase are confirmed to be involved in heme/hemoglobin utilization in C. jejuni.
    • Evidence suggests the presence of additional inner membrane ABC transporters for heme uptake.
    • The heme uptake system is regulated by iron availability through Fur-dependent mechanisms.