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Related Experiment Video

Updated: Jul 20, 2026

Yeast As a Chassis for Developing Functional Assays to Study Human P53

Yeast As a Chassis for Developing Functional Assays to Study Human P53

Published on: August 4, 2019

Functional analysis of p53 binding under differential stresses.

Adam J Krieg¹, Ester M Hammond, Amato J Giaccia

¹Division of Radiation and Cancer Biology, Department of Radiation Oncology, Stanford University, Stanford, CA 94303-5152, USA.

Molecular and Cellular Biology

|September 19, 2006

Summary

Different stresses stabilize the p53 protein, but gene expression varies. This study identified novel p53 target genes and found distinct expression patterns under hypoxia or DNA damage.

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Area of Science:

Molecular Biology
Genomics
Cancer Research

Background:

The tumor suppressor protein p53 is stabilized by cellular stresses like hypoxia and DNA damage.
The transcriptional response of p53 target genes to these stresses is known to be variable.

Purpose of the Study:

To identify novel p53 target genes bound by p53 under both hypoxia and DNA-damaging conditions.
To investigate the differential expression patterns of these target genes in response to distinct stress stimuli.

Main Methods:

Chromatin immunoprecipitation followed by CpG island (CGI) microarray hybridization was used to identify p53-bound promoters.
Gene-specific PCR analysis validated the enrichment of p53 binding at specific CGIs.
Analysis of p53 mutated in the DNA binding domain explored binding mechanisms.

Main Results:

Little difference was observed in p53 promoter binding between hypoxia and DNA damage.
Identified novel p53 target genes including REV3L, MYC, and PCNA.
Gene expression varied significantly, with most genes showing no change or repression, rather than induction, under stress.

Conclusions:

p53 interacts with CGIs through both sequence-dependent and -independent mechanisms.
Novel p53 target genes were identified.
Distinct stress-induced expression phenomena for p53 target genes were discovered, highlighting context-dependent regulation.