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Updated: Jul 20, 2026

Visualization of ATP Synthase Dimers in Mitochondria by Electron Cryo-tomography
Published on: September 14, 2014
gammaepsilon Sub-complex of thermophilic ATP synthase has the ability to bind ATP
Satoshi Iizuka1, Shigeyuki Kato, Masasuke Yoshida
1Department of Life Science, College of Science, Rikkyo University, 3-34-1, Nishi-Ikebukuro, Tokyo 171-8501, Japan.
Abstract:
The isolated epsilon subunit of F(1)-ATPase from thermophilic Bacillus PS3 (TF(1)) binds ATP [Y. Kato-Yamada, M. Yoshida, J. Biol. Chem. 278 (2003) 36013]. The obvious question is whether the ATP binding concern with the regulation of ATP synthase activity or not. If so, the epsilon subunit even in the ATP synthase complex should have the ability to bind ATP. To check if the ATP binding to the epsilon subunit within the ATP synthase complex may occur, the gammaepsilon sub-complex of TF(1) was prepared and ATP binding was examined. The results clearly showed that the gammaepsilon sub-complex can bind ATP.
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