[In vitro screening of 32 traditional Chinese herbal extracts against U2OS human osteosarcoma cells]
Jin Wang1, Jun-qiang Yin, Jing-nan Shen
1Department of Musculoskeletal Oncology, First Affiliated Hospital of Sun Yat-sen University, Guangzhou 510080, China. wjhf@hotmail.com
Objective:
To test 32 traditional Chinese medicinal herbs with effects against osteosarcoma in vitro.
Methods:
U(2)OS human osteosarcoma cell line was treated with the extracts of the Chinese herbs at various concentrations. The changes in cell proliferation in response to the treatment were examined by MTT assay, and the effects of these extracts against human osteosarcoma growth were compared. Morphological observation, flow cytometry and Annexin V were employed to detect the cell apoptosis after the treatments.
Results:
According to the results of MTT assay, several of the 32 Chinese herbs, especially Venenum Bufonis and oxgall powder, were identified to produce growth inhibition against U(2)OS cells. Further study of the aqueous extracts of Venenum Bufonis and oxgall powder demonstrated their effects in inducing U(2)OS cell apoptosis, and Venenum Bufonis showed the strongest effect. In spite of the obvious growth inhibitory effect of oxgall powder, its extract induced cell apoptosis only at high concentrations.
Conclusion:
Several of the traditional Chinese herbs, especially Venenum Bufonis and oxgall powder, may inhibit the growth of U(2)OS cell line, and the results of this study pave the way for further identification of the effective components in the herbs that inhibit osteosarcoma growth both in vivo and in vitro.
Insights
Venenum Bufonis and oxgall powder show potential in inhibiting osteosarcoma cell growth. Further research into these traditional Chinese herbs may lead to new treatments for osteosarcoma.
Area of Science:
- Traditional Chinese Medicine
- Oncology
- Pharmacology
Context:
- Osteosarcoma is a primary bone cancer with limited treatment options.
- Traditional Chinese Medicine (TCM) offers a rich source of compounds for drug discovery.
- Investigating TCM for anti-cancer properties is crucial for developing novel therapeutics.
Purpose:
- To evaluate the in vitro efficacy of 32 TCM herbs against human osteosarcoma cells (U(2)OS).
- To identify specific TCM herbs that inhibit osteosarcoma cell proliferation and induce apoptosis.
- To compare the anti-cancer effects of selected TCM extracts on osteosarcoma.
Summary:
- 32 TCM herbs were screened for anti-osteosarcoma activity using U(2)OS cells.
- Venenum Bufonis and oxgall powder demonstrated significant inhibition of U(2)OS cell growth.
- Venenum Bufonis extract induced apoptosis effectively, while oxgall powder required higher concentrations.
Impact:
- Identifies Venenum Bufonis and oxgall powder as promising candidates for osteosarcoma treatment.
- Provides a foundation for isolating active components from these TCM herbs.
- Supports the potential of TCM in developing novel anti-osteosarcoma therapies.
