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Updated: Jul 20, 2026
Generation of Transgenic Rats using a Lentiviral Vector Approach
Published on: May 17, 2020
[Construction and identification of tetracycline-inducible rat Smad7 eukaryotic expression vector]
Shu-ting Ren1, Lin-hua Yu, Chang-fu Xu
1Department of Pathology, Medical College of Xi'an Jiaotong University, Xi'an 710061, China. rst@mail.xjtu.edu.cn
Objective:
To construct a tetracycline-inducible eukaryotic expression vector of rat Smad7.
Methods:
The total RNA was extracted from normal rat kidney with Trizol agent. Rat Smad7 cDNA fragment was cloned by RT-PCR, and was inserted into the restriction site between Nhe I and Hind III of the inducible eukaryotic expression vector pBI-L by tetracycline. pBI-L-Smad7 was constructed by digestion and ligation, and detected by restriction endonuclease digestion and sequencing.
Results:
The recombinant eukaryotic expression vector pBI-L-Smad7 was constructed correctly as confirmed by restriction endonuclease digestion and sequencing. The fragment of pBI-L-Smad7 digested with restriction endonucleases and the sequence of inserted Smad7 cDNA were consistent with the results of theoretical analysis.
Conclusion:
The tetracycline- inducible eukaryotic expression vector of rat Smad7, pBI-L-Smad7, is constructed successfully, which may facilitate further clinical study of Smad7 gene therapy for tissue and organ fibrosis.
