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Updated: Jul 20, 2026
Multiplexed Isothermal Amplification Based Diagnostic Platform to Detect Zika, Chikungunya, and Dengue 1
Published on: March 13, 2018
[Detection and typing of dengue virus using polymerase chain reaction and microwell plate hybridization]
Rui-wen Ren1, Xiao-li Xu, Jian-jun Li
1Disease Control and Prevention Center of Guangzhou Command, Guangzhou 510507, China. renruiwen@hotmail.com
Objective:
To establish a specific, sensitive and practicable method for detection and typing of dengue virus.
Methods:
Based on the genomic sequence analysis of dengue virus types 1-4, 4 pairs of primers were designed. The specific capture probes of dengue virus types 1-4 were amplified using RT-PCR, cloned and sequenced before using them for precoating the microwell plate. The samples were amplified using biotin-labeled forward primer and reverse primer, and microwell plate hybridization was carried out for detection and typing of dengue virus types 1-4.
Results:
The absorbance of the positive samples were higher than 0.5, while the average absorbance of the negative samples was lower than 0.1, with the S/N higher than 10.
Conclusion:
The method of PCR-ELISA we established for early detection and typing of all 4 dengue viruses seretypes.
