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Updated: Jul 20, 2026
Isolation of Murine Peritoneal Macrophages to Carry Out Gene Expression Analysis Upon Toll-like Receptors Stimulation
Published on: April 29, 2015
[Effect of resveratrol on lipopolysaccharide-induced activation of rat peritoneal macrophages]
Zhen-hua Ma1, Qing-yong Ma, Huan-chen Sha
1Department of Hepatobiliary Surgery, First Hospital of Xi'an Jiaotong University, Xi'an 710061, China.
Objective:
To investigate nuclear factor kappa B (NF-kappaB) activation induced by lipopolysaccharide (LPS) in rat peritoneal macrophages (PMAs) and the inhibitory effect of resveratrol on NF-kappaB activation.
Methods:
PMAS from normal SD rats were randomly divided into 7 groups, including a control group, a LPS group and 5 resveratrol groups (I-V). PMAs of the control group were incubated in DMEM, and those in LPS group in DMEM containing LPS (10 microg/ml). PMAS of resveratrol groups I-V were incubated in DMEM containing LPS (10 microg/ml) and different concentrations of resveratrol. After 24 h of incubation, NF-kappaB activation in the PMAs was determined, and the expression levels of tumor necrosis factor alpha (TNF-alpha), interleukin-1 (IL-1) and nitric oxide (NO) in the culture medium were measured.
Results:
Exposure to LPS resulted in an excessive enhancement of cytokine and NO expressions in the PMAs. Resveratrol at 1.25-10 microg/ml produced a dose- dependent inhibition of cytokine and NO expressions and on NF-kappaB activation in LPS-stimulated PMAs.
Conclusion:
Resveratrol can inhibit LPS-induced NF-kappaB activation in rat PMAs and subsequently suppress the expressions of TNF-alpha, IL-1 and NO.
Insights
Resveratrol effectively inhibits lipopolysaccharide-induced nuclear factor kappa B (NF-kappaB) activation in rat macrophages. This natural compound suppresses key inflammatory markers like tumor necrosis factor alpha and nitric oxide.
Area of Science:
- Immunology and Molecular Biology
- Pharmacology
Background:
- Lipopolysaccharide (LPS) is a potent activator of inflammatory responses in macrophages.
- Nuclear Factor kappa B (NF-kappaB) is a critical transcription factor regulating inflammatory gene expression.
- Resveratrol, a natural polyphenol, has demonstrated anti-inflammatory properties.
Purpose of the Study:
- To examine NF-kappaB activation in rat peritoneal macrophages (PMAs) stimulated by LPS.
- To evaluate the inhibitory effects of resveratrol on LPS-induced NF-kappaB activation and subsequent inflammatory mediator production.
Main Methods:
- Rat PMAs were cultured and exposed to LPS and varying concentrations of resveratrol.
- NF-kappaB activation was assessed.
- Levels of tumor necrosis factor alpha (TNF-alpha), interleukin-1 (IL-1), and nitric oxide (NO) in the culture supernatant were quantified.
Main Results:
- LPS exposure significantly increased TNF-alpha, IL-1, and NO expression in PMAs.
- Resveratrol demonstrated a dose-dependent inhibition of these inflammatory markers.
- Resveratrol suppressed NF-kappaB activation in LPS-stimulated PMAs.
Conclusions:
- Resveratrol effectively inhibits LPS-induced NF-kappaB activation in rat PMAs.
- Resveratrol suppresses the production of key inflammatory mediators, including TNF-alpha, IL-1, and NO.
- These findings highlight resveratrol's potential as an anti-inflammatory agent.
