Combined staining of acidophilic inclusion bodies and lipofuscin granules

Gomori's trichrome solution is shown to stain intracytoplasmic acidophilic granules that occur in some neuronal types of the human brain. Since nerve cells in the adult human brain can be classified on account of their characteristic pattern of lipofuscin pigmentation, a combined staining method is described for the demonstration of both lipofuscin granules and intracytoplasmic acidophilic granules. Paraffin sections are stained with aldehyde fuchsin and Gomori's trichrome solution. In this manner, the red acidophilic granules are in sharp contrast to the lipofuscin deposits stained in a bluish shade. Due to this staining procedure it is now possible to classify neurons revealing acidophilic granules as well as to determine their exact topographical localization.