Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

A new approach to study fibroblast migration.

Bhavani P Thampatty1, James H-C Wang

  • 1MechanoBiology Laboratory, Departments of Orthopaedic Surgery, Bioengineering, and Mechanical Engineering, University of Pittsburgh, 210 Lothrop Street, Pittsburgh, PA 15213, USA.

Cell Motility and the Cytoskeleton
|September 21, 2006
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Repurposing Metformin to Promote Fracture Callus Maturation via AMPK-Driven Metabolic Activation.

Journal of orthopaedic research : official publication of the Orthopaedic Research Society·2026
Same author

Impact of porcine skin processing on in vitro permeation testing: implications for reliable evaluation of topical metformin delivery.

European journal of pharmaceutical sciences : official journal of the European Federation for Pharmaceutical Sciences·2026
Same author

Injectable hyaluronic acid-metformin conjugate gel for sustained intra-articular delivery and prevention of post-traumatic osteoarthritis.

Bioengineering & translational medicine·2026
Same author

Comparative human and porcine skin permeation profiles of novel metformin lotion formulations.

Scientific reports·2025
Same author

Metformin lotion as a novel approach to prevent tendinopathy induced by mechanical overuse.

Scientific reports·2025
Same author

Quantifying skin permeation of a novel metformin lotion using modified hydrophilic interaction liquid chromatography.

Bioanalysis·2024

This study introduces microgrooved surfaces for studying cell migration. These surfaces guide human tendon fibroblasts, making their movement easier to quantify and analyze, especially after myofibroblast differentiation.

Area of Science:

  • Biomedical Engineering
  • Cell Biology
  • Tissue Engineering

Background:

  • Cell migration is crucial for tissue development and repair.
  • Quantifying cell motility on conventional surfaces is challenging due to random, two-dimensional movement.
  • Developing standardized methods for cell migration analysis is essential.

Purpose of the Study:

  • To present a novel microgrooved surface approach for studying cell migration.
  • To investigate the behavior and motility of human tendon fibroblasts on microgrooved substrates.
  • To assess the impact of transforming growth factor-beta1 (TGF-β1) on fibroblast differentiation and migration.

Main Methods:

  • Human tendon fibroblasts were cultured on ProNectin-F coated silicone membranes with microgrooves (10 µm width, 3 µm depth).

Related Experiment Videos

  • Cell morphology, orientation, and migration patterns were observed on microgrooved versus smooth surfaces.
  • Fibroblast differentiation into myofibroblasts was induced using TGF-β1 (5 ng/ml), and alpha-smooth muscle actin (α-SMA) expression was measured.
  • Cell motility was quantified by measuring migration speed on microgrooved surfaces.

    • Main Results:

    • Fibroblasts on microgrooves exhibited elongated shapes and oriented migration along the groove direction.
    • TGF-β1 treatment induced myofibroblast differentiation, marked by increased α-SMA expression.
    • Myofibroblasts on microgrooved surfaces showed approximately 30% reduced motility compared to untreated fibroblasts.
    • Microgrooved surfaces facilitated one-dimensional cell migration, simplifying quantification.

    Conclusions:

    • Microgrooved surfaces provide a controlled environment for studying cell migration.
    • This approach enables more accurate quantification of cell motility compared to traditional methods.
    • The microgroove system can be used to study changes in cell behavior, such as differentiation-induced motility reduction.