Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Reverse transcriptase activity from human embryonal carcinoma cells NTera2D1.

J M Deragon1, D Sinnett, D Labuda

  • 1Service de Génétique Médicale, Hôpital Ste-Justine, Université de Montréal, Québec, Canada.

The EMBO Journal
|October 1, 1990
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Genetic factors contributing to late adverse musculoskeletal effects in childhood acute lymphoblastic leukemia survivors.

The pharmacogenomics journal·2021
Same author

Pre-implantation alcohol exposure induces lasting sex-specific DNA methylation programming errors in the developing forebrain.

Clinical epigenetics·2021
Same author

Nutrition education and cooking workshops for families of children with cancer: a feasibility study.

BMC nutrition·2020
Same author

Developmental genome-wide DNA methylation asymmetry between mouse placenta and embryo.

Epigenetics·2020
Same author

Impact of DARC, GSDMA and CXCL2 polymorphisms on induction toxicity in children with acute lymphoblastic leukemia: A complementary study.

Leukemia research·2019
Same author

Environmental, health, wellbeing, social and equity effects of urban green space interventions: A meta-narrative evidence synthesis.

Environment international·2019
Same journal

Scalable phosphotyrosine enrichment with SH2 superbinder enables deep profiling of EGF responses.

The EMBO journal·2026
Same journal

Essential nucleus-apical pole linkage maintains division fidelity during Plasmodium progeny formation.

The EMBO journal·2026
Same journal

From cell atlases to mechanisms: bridging scRNA-seq discovery with in vivo genetics.

The EMBO journal·2026
Same journal

Mitochondrial calcium regulates lipid metabolism by modulating tethering of mitochondria to lipid droplets.

The EMBO journal·2026
Same journal

Chromosome condensation mechanically primes the nucleus for mitosis.

The EMBO journal·2026
Same journal

NDR kinase SAX-1 controls dendrite branch-specific elimination during neuronal remodeling in C. elegans.

The EMBO journal·2026
See all related articles

Researchers found reverse transcriptase (RT) activity in human embryonal carcinoma cells. UV irradiation enhanced this activity, which was purified and associated with viral-like particles, potentially linked to Line-1 elements and retroposition.

Area of Science:

  • Molecular Biology
  • Genetics
  • Cell Biology

Background:

  • Human embryonal carcinoma cells (NTera2D1) express full-length genomic Line-1 (L1) elements.
  • RNA-dependent DNA polymerase activity, or reverse transcriptase (RT), is crucial for retrotransposition.

Purpose of the Study:

  • To identify and characterize reverse transcriptase activity in NTera2D1 cells.
  • To investigate the potential role of RT in Line-1 element activity.

Main Methods:

  • Microsomal fraction isolation from NTera2D1 cells.
  • Enzyme assays using a synthetic poly(Cm) template and Mn2+.
  • Purification via glycerol step gradient, sucrose density gradient, and Sephacryl S-1000 gel filtration.
  • UV irradiation treatment to enhance RT detection.

Related Experiment Videos

Main Results:

  • Identified and characterized RNA-dependent DNA polymerase (RT) activity in NTera2D1 cells.
  • RT activity was significantly enhanced (2-3 fold) by UV irradiation.
  • Purification yielded a >100-fold enrichment of RT activity associated with viral-like particles (37 kd protein).
  • L1 mRNA was detected in RT-containing fractions.

Conclusions:

  • The identified RT activity likely originates from or is involved in the mechanism of Line-1 retroposition.
  • RT activity in these cells is associated with viral-like particles.
  • UV irradiation is essential for reproducible detection of this RT activity.