[Comparison of TK gene mutation assay in TK6 and TK6-E6 cell induced by vinblastin and colcemid]

To compare TK gene mutation assay in human lymphoblastoid cell lines TK6 and TK6-E6 induced by vinblastin and colcemid.

Regular TK gene mutation assays were experimented to detect cytotoxicity and mutation frequency at tk locus after TK6 and TK6-E6 human lymphoblastoid cells were treated with vinblastin and colcemid for 24h.

Relative survival (RS%) and Relative suspension growth (RSG%) of TK6 and TK6-E6 cells decreased when concentrations of vinblastin and colcemid increased. RS% and RSG% of TK6-E6 cells were higher than that of TK6 cells at the same doses. TK6 and TK6-E6 cells both were induced by vinblastin and colcemid. The TK6-E6's nature mutation frequency, induced mutation frequency and percentage of slow growth mutant (SG%) were lower than TK6' s at the same dose except group CCM 5.0ng/ml in MF and group CCM 0.625ng/ml in SC%.

Both TK6 and TK6-E6 cell lines can be used in TK gene mutation assay, but TK6-E6 cell line was recommended to be used in the assay for its less quantity and doubling time.