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Updated: Jul 19, 2026

A Protocol for the Production of KLRG1 Tetramer
Published on: January 12, 2010
[Production and characterization of gold labeled monoclonal antibody against MC-LR]
Xiao-lian Zhao1, Wei-rong Sun, Xiu-lan Sun
1Southern Yangtz University, Wuxi 214036, China.
Objective:
Production of the gold labeled monoclonal antibody against MC-LR. Study the simple mechanism of the conjugation by the spectral analysis.
Methods:
Production and purification monoclonal antibodies against MC-LR by hybridoma technology. Production the 20nm colloidal gold and label the monoclonal antibody. Study the gold--McAb conjugate by the spectroanalysis.
Results:
Monoclonal antibody produced by the hybridoma cells were tested for subtypes and designated as IgG2a for 8C11. The titers of antibody in ascites was 108.The IC50 for MC-LR was around 3ng/ml. The IgG molecule weight was 1.5 x 10(5). The structure of the antibody was altered to more tighter and stabler.
Conclusion:
Product the gold labeled monoclonalantibody against MC-LR conjugate successfully and keep the antige-binding determinant.
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