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Updated: Jul 19, 2026
09:53
A Purification and In Vitro Activity Assay for a (p)ppGpp Synthetase from Clostridium difficile
Published on: November 3, 2018
Application of mutated Clostridium difficile toxin A for determination of glucosyltransferase-dependent effects
Matthias Teichert1, Helma Tatge, Janett Schoentaube
1Institut für Toxikologie, Medizinische Hochschule Hannover, Carl-Neuberg-Str. 1, 30625 Hannover, Germany.
Infection and Immunity
|September 22, 2006
Abstract:
Mutation of tryptophan-101 in Clostridium difficile toxin A, a 308-kDa glucosyltransferase, resulted in a 50-fold-reduced cytopathic activity in cell culture experiments. The mutant toxin A was characterized and applied to distinguish between glucosyltransferase-dependent and -independent effects with respect to RhoB up-regulation as a cellular stress response.
