Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Nucleic acid extraction from Agrobacterium strains.

Arlene A Wise1, Zhenying Liu, Andrew N Binns

  • 1Plant Science Institute, Department of Biology, University of Pennsylvania, PA, USA.

Methods in Molecular Biology (Clifton, N.J.)
|September 22, 2006
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Widely-targeted metabolomics reveals phased metabolic response underlying light-induced quality deterioration of dried Dendrobium huoshanense petals in storage.

Plant physiology and biochemistry : PPB·2026
Same author

UPLC-ESI-QTRAP-MS/MS identification and antioxidant activity of hawthorn with different infestation levels.

BMC chemistry·2025
Same author

Insights into the quality profile and potential quality markers of <i>Lycium barbarum</i> (goji) berries after <i>Tribolium castaneum</i> infestation.

Food chemistry: X·2025
Same author

Comprehensive Analysis on Physicochemical Properties and Characteristic Compounds of Insect-Infested Ziziphi Spinosae Semen.

Metabolites·2025
Same author

N-acetylation of α-synuclein enhances synaptic vesicle clustering mediated by α-synuclein and lysophosphatidylcholine.

eLife·2024
Same author

Unveiling the potential applications of buds of <i>Lonicera japonica</i> Thunb. var. <i>chinensis</i> (Wats.) Bak based on <i>in vitro</i> biological activities, bio-active components, and potential applications coupled to targeted metabolomics.

Frontiers in plant science·2024

This study presents reliable methods for isolating DNA and RNA from Agrobacterium tumefaciens, crucial for plant genetic engineering. It also details a technique for transferring plasmids to E. coli when needed.

Area of Science:

  • Molecular Biology
  • Plant Biotechnology
  • Microbiology

Background:

  • Agrobacterium tumefaciens is a key tool for plant genetic engineering.
  • Successful genetic modification requires efficient manipulation of nucleic acids within Agrobacterium.
  • Existing methods for nucleic acid isolation and plasmid transfer can be limiting.

Purpose of the Study:

  • To provide robust protocols for isolating various nucleic acid types from Agrobacterium.
  • To develop a method for transferring plasmids from Agrobacterium to E. coli.
  • To facilitate genetic engineering applications in plant science.

Main Methods:

  • Genomic DNA isolation
  • Mega-plasmid DNA isolation
  • Shuttle/binary plasmid DNA isolation

Related Experiment Videos

  • RNA isolation
  • Electroporation-based plasmid transfer to E. coli

    • Main Results:

    • Dependable methods for isolating genomic DNA, mega-plasmid DNA, shuttle plasmid DNA, and RNA were established.
    • A simple and effective method for the electronic transfer of shuttle plasmids to E. coli was demonstrated.
    • These methods address challenges associated with low copy number plasmids in Agrobacterium.

    Conclusions:

    • The presented methods enhance the utility of Agrobacterium in plant genetic engineering.
    • Efficient nucleic acid isolation and plasmid transfer are critical for advancing plant biotechnology.
    • These protocols offer valuable tools for researchers in molecular biology and plant science.