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Transgenic Plants

Transgenic Plants

Recombinant DNA technology called transgenesis is often used to add a foreign gene or remove a detrimental gene from an organism. Such genetically modified organisms are called transgenic organisms.
The first-ever transgenic plant was a tobacco plant developed in 1983 that showed resistance against the tobacco mosaic virus. Since then, many transgenic plants have been developed and commercialized for improving the agricultural, ornamental, and horticultural value of a crop plant. Transgenic...

Transgenic Organisms

Transgenic Organisms

Plant Tissue Culture

Plant Tissue Culture

Plant tissue culture is widely used in both primary and applied science. Applications range from plant development studies to functional gene studies, crop improvement, commercial micropropagation, virus elimination, and conservation of rare species.

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Crop cultivation has a long history in human civilization, with records showing the cultivation of cereal plants beginning at around 8000 BC. This early plant breeding was developed primarily to provide a steady supply of food.

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Related Experiment Video

Updated: Jul 19, 2026

An Efficient and Reproducible Method for Producing Composite Plants by Agrobacterium rhizogenes-Based Hairy Root Transformation

An Efficient and Reproducible Method for Producing Composite Plants by Agrobacterium rhizogenes-Based Hairy Root Transformation

Published on: June 30, 2023

Generation of composite plants using Agrobacterium rhizogenes.

Christopher G Taylor¹, Beth Fuchs, Ray Collier

¹Donald Danforth Plant Science Center, St. Louis, MO, USA.

Methods in Molecular Biology (Clifton, N.J.)

|September 22, 2006

Summary

Developing transgenic roots on plant shoots, known as composite plants, overcomes transformation barriers. This method enables efficient gene function studies in various plant species, including difficult-to-transform ones.

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Last Updated: Jul 19, 2026

An Efficient and Reproducible Method for Producing Composite Plants by Agrobacterium rhizogenes-Based Hairy Root Transformation

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Published on: June 30, 2023

Generation of Composite Plants in Medicago truncatula used for Nodulation Assays

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Published on: March 27, 2011

Agrobacterium tumefaciens and Agrobacterium rhizogenes-Mediated Transformation of Potato and the Promoter Activity of a Suberin Gene by GUS Staining

Agrobacterium tumefaciens and Agrobacterium rhizogenes-Mediated Transformation of Potato and the Promoter Activity of a Suberin Gene by GUS Staining

Published on: March 29, 2019

Area of Science:

Plant biotechnology
Molecular biology
Genetics

Background:

Transformation efficiency is a key limitation in understanding plant gene function.
Transgenic approaches are crucial for advancing plant science and crop improvement.

Purpose of the Study:

To develop robust methodologies for generating transgenic composite plants.
To enable efficient gene function analysis within the context of a whole plant.

Main Methods:

Developed tissue culture and non-tissue culture methods for creating composite plants using Agrobacterium rhizogenes.
Inoculated wild-type shoots to induce transgenic root formation on various dicotyledonous species.
Established protocols for Arabidopsis and other recalcitrant plant species.

Main Results:

Successfully generated transgenic composite plants using both tissue culture and non-tissue culture approaches.
Demonstrated the efficiency of the methods across diverse plant species, including those with transformation challenges.
Composite plants could be grown and analyzed like normal plants in standard greenhouse conditions.

Conclusions:

The developed composite plant system effectively bypasses transformation limitations.
This methodology provides a versatile tool for gene function studies and plant-microbe interactions.
The methods are applicable to a wide range of plant species, enhancing genetic research capabilities.