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Related Experiment Video

Updated: Jul 2, 2026

Isolation of Histone from Sorghum Leaf Tissue for Top Down Mass Spectrometry Profiling of Potential Epigenetic Markers
07:10

Isolation of Histone from Sorghum Leaf Tissue for Top Down Mass Spectrometry Profiling of Potential Epigenetic Markers

Published on: March 4, 2021

Sorghum (Sorghum bicolor L.).

Zuo-yu Zhao1

  • 1Crop Genetics Research and Development, Pioneer Hi-Bred International, Inc., A DuPont Company, Johnston, IA, USA.

Methods in Molecular Biology (Clifton, N.J.)
|September 22, 2006
PubMed
Summary

This study details a method for Agrobacterium-mediated genetic transformation in sorghum using immature embryos. The protocol facilitates the creation of transgenic sorghum plants with selectable marker-free trait genes.

Area of Science:

  • Plant Science
  • Biotechnology
  • Genetics

Background:

  • Agrobacterium-mediated transformation is a key tool for plant genetic improvement.
  • Developing efficient transformation protocols for staple crops like sorghum is crucial for food security.
  • Eliminating selectable marker genes in transgenic crops is desirable for regulatory and commercial reasons.

Purpose of the Study:

  • To describe a stepwise protocol for Agrobacterium-mediated genetic transformation of sorghum.
  • To introduce a co-transformation strategy for generating selectable marker-free transgenic sorghum.
  • To provide guidelines for analyzing transgenic sorghum and confirming gene integration and segregation.

Main Methods:

  • Utilized immature sorghum embryos as explants for transformation.

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  • Employed Agrobacterium strain LBA4404 with a super-binary vector.
  • Implemented a co-transformation approach with separate T-DNAs for selectable marker and trait genes.
  • Main Results:

    • Successfully established a protocol for sorghum genetic transformation.
    • Demonstrated the use of co-transformation vectors for marker-free trait gene introduction.
    • Provided methods for confirming T-DNA integration and segregation in transgenic sorghum.

    Conclusions:

    • The described protocol enables efficient Agrobacterium-mediated transformation of sorghum.
    • The co-transformation strategy allows for the development of marker-free transgenic sorghum lines.
    • Recommended analytical methods ensure the genetic integrity and desired trait expression in transformed sorghum.