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Related Experiment Video

Updated: Jul 19, 2026

Assessing Structural Traits in Triticum aestivum and Zea mays for C3 and C4 Photosynthetic Differentiation Using Free-hand and Semi-thin Sections

Assessing Structural Traits in Triticum aestivum and Zea mays for C₃ and C₄ Photosynthetic Differentiation Using Free-hand and Semi-thin Sections

Published on: July 12, 2024

Wheat (Triticum aestivum L.).

Yuechun Wan¹, Jeanne Layton

¹Monsanto Company, Agracetus Campus, Middleton, WI, USA.

Methods in Molecular Biology (Clifton, N.J.)

|September 22, 2006

Summary

This study details a reliable Agrobacterium-mediated method for creating transgenic wheat. The efficient protocol yields morphologically normal, seed-producing R0 plants, with many exhibiting single-copy transgene integration.

Area of Science:

Plant Biotechnology
Molecular Biology
Agricultural Science

Background:

Agrobacterium-mediated transformation is a key tool for genetic modification in plants.
Efficiently generating transgenic wheat lines is crucial for crop improvement.

Purpose of the Study:

To describe a detailed procedure for Agrobacterium-mediated wheat transformation.
To establish a reproducible method for generating genetically modified wheat.

Main Methods:

Inoculation of wheat explants (immature embryos or calli) with Agrobacterium tumefaciens strain C58 (ABI) carrying a binary vector with GUS and NPT II genes.
Selection of transformed tissues on G418-containing medium.
Regeneration of plants from selected calli under reduced selective pressure and plant growth regulators.

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Main Results:

Successful generation of transgenic wheat (R0 plants) within 2.5 to 3 months post-inoculation.
Transgenic plants were morphologically normal and fertile, producing seeds.
Approximately 35% of confirmed transgenic plants possessed a single copy of the integrated transgene.

Conclusions:

The described Agrobacterium-mediated protocol provides an effective route for wheat transformation.
The method yields viable, fertile transgenic wheat lines suitable for further research and breeding.
The efficiency and characteristics of the resulting transgenic plants support its utility in wheat genetic studies.